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2025年4月6日 星期日
农业生物技术学报  2023, Vol. 31 Issue (3): 445-459    DOI: 10.3969/j.issn.1674-7968.2023.03.001
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
小麦PDR基因家族的鉴定与表达分析
何浩, 王志明, 张立强, 唐艺真, 夏琪, 李忠源, 张怀渝*
四川农业大学 生命科学学院,雅安 625014
Identification and Expression Analysis of PDR Gene Family in Wheat (Triticum aestivum)
HE Hao, WANG Zhi-Ming, ZHANG Li-Qiang, TANG Yi-Zhen, XIA Qi, LI Zhong-Yuan, ZHANG Huai-Yu*
College of Life Sciences, Sichuan Agricultural University, Ya'an 625014
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摘要 多向耐药性蛋白(pleiotropic drug resistance, PDR)是ABC转运蛋白超家族重要的亚家族成员,在响应外界胁迫和植物生长发育的生理过程中发挥着重要作用,但在小麦(Triticum aestivum)中研究较少。为探讨小麦PDR基因家族对逆境胁迫的响应,本研究对小麦PDR基因家族进行了全基因组鉴定和生物信息学分析,并通过qPCR方法验证其在非生物胁迫下的响应情况。结果显示,小麦中共鉴定出76个TaPDRs基因,主要位于3、5和7号染色体上,编码氨基酸1 242~1 517个,分子量为140.69~170.99 kD,预测等电点范围为6.22~8.94;系统进化分析将TaPDRs基因分为5类(ClassⅠ~Ⅴ),多数TaPDRs蛋白基序具有高度的保守性,同一类型TaPDRs具有较为相似的基因结构;基因复制分析共发现10组串联复制基因和56对片段复制基因;组织特异性分析发现,TaPDRs基因在小麦不同组织中的表达水平有较大差异,大多在根组织中高表达;qPCR结果显示,TaPDRs基因能够不同程度的响应盐、干旱、镉胁迫以及脱落酸处理。本研究为探索TaPDRs基因在非生物胁迫响应中的作用提供参考。
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何浩
王志明
张立强
唐艺真
夏琪
李忠源
张怀渝
关键词 小麦PDR基因家族生物信息学分析非生物胁迫    
Abstract:Pleiotropic drug resistance (PDR) is an important subfamily member of the ABC transporter superfamily, which plays important role in response to stress, plant growth and development, but little research has been done in wheat (Triticum aestivum). In order to investigate the response of wheat PDR gene family to stress, genome-wide identification and bioinformatics analysis of TaPDR gene family were performed in this study, and the responses to abiotic stress were verified by qPCR. The results showed that 76 TaPDR genes were mainly distributed on chromosome 3, 5 and 7. The number of coding amino acids was 1 242~1 517, and the molecular weight was 140.69~170.99 kD, pI was between 6.22 and 8.94. According to phylogenetic analysis, TaPDR genes were divided into 5 groups (Class Ⅰ~Ⅴ), most of TaPDRs had highly conserved protein motifs, and TaPDR genes in the same group had more similar gene structure. 10 pairs of tandem repeat genes and 56 pairs of segmental duplication genes were founded with gene duplication analysis method. The tissue-specific analysis showed that TaPDR genes exhibited different expression levels in different tissues. Most TaPDR genes were highly expressed in roots. qPCR results showed that TaPDR genes could respond to salt, drought, cadmium and abscisic acid treatment in different degree. The results provide a reference basis for the mechanism of TaPDR gene family in abiotic stress response.
Key wordsWheat    PDR gene family    Bioinformatics analysis    Abiotic stress
收稿日期: 2022-04-02     
ZTFLH:  S512.1  
基金资助:国家自然科学基金(31971940)
通讯作者: * zhyu@sicau.edu.cn   
引用本文:   
何浩, 王志明, 张立强, 唐艺真, 夏琪, 李忠源, 张怀渝. 小麦PDR基因家族的鉴定与表达分析[J]. 农业生物技术学报, 2023, 31(3): 445-459.
HE Hao, WANG Zhi-Ming, ZHANG Li-Qiang, TANG Yi-Zhen, XIA Qi, LI Zhong-Yuan, ZHANG Huai-Yu. Identification and Expression Analysis of PDR Gene Family in Wheat (Triticum aestivum). 农业生物技术学报, 2023, 31(3): 445-459.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2023.03.001     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2023/V31/I3/445
 
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