Abstract：The storability of rice (Oryza sativa) seeds is an important guarantee for food security and the safety of seed use. It is the basis of genetic improvement of rice seed storability to explore QTL loci and screen excellent storability haplotypes. In this study, 431 core collections from 3 000 rice (3K rice) resources in the world were used as research materials, which were sown in Changsha, Hunan in 2019 and in Sanya, Hainan in 2020, making the maturity period of each trial material basically consistent. The harvested seeds were identified for storability after natural storage and artificial aging treatment. Genome wide association study (GWAS) was performed on rice seed storability based on genotypic data. Using P<1×10-4 as the threshold, 14 QTL for seed storability were repeatedly detected, which were distributed on chromosome 2, 3, 5, 8, 9, 10, 11 and 12. The explained phenotypic variation ranged from 5% to 24%, and qSS3-2 and qSS5-2 were new QTL loci for seed storability. A total of 17 candidate genes related to rice seed storage tolerance were mined in these 14 QTL intervals, and haplotype analysis of the candidate genes in the qSS5-2 interval yielded 8 rice seed storability haplotypes. The results of this study provides theoretical reference and important gene resources for genetic improvement of rice seed storability.
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