Abstract：Proteins containing steroidogenic acute regulatory protein-related lipid transfer (START) domain exist widely in the biological world, and are generally divided into 2 classes, one containing both the START domain and other domains, and the other containing the START domain only. In Arabidopsis thaliana, there are 35 proteins containing START domain, 8 of them only have START domain, and this protein subfamily containing START domain only provides a good research material for the biological function of START domain. This study analyzed the tissue expression characteristics of the 8 members by qPCR and bioinformatics method. The results showed that 1st, 3rd, 4th, and 5th chromosome each had 2 of the 8 members, and the size of the START domain and the amino acid sequence of the 8 members were not exactly the same. The 8 members had different tissue expression. In order to clarify the biological function of START domain, the gene overexpression plants and T-DNA insertion mutants of the 8 members were used as materials, the phenotypes of seedlings, leaves and pods were analyzed. Gene overexpression plants and T-DNA insertion mutants of subfamily members containing only the START domain showed asymmetric leaf growth, curling and arrested development. At the same time, the pod was no longer in whorl growth, the pod initiation became disordered, and there was a continuous growth on the same side of the stem. The above results indicate that the START domain family plays an important role in plant morphogenesis. The present study provides basic material for elucidating the molecular regulating mechanism of START domain in plant growth and development.
刘霞, 李晓凯, 常红娟, 刘彤彤, 王凤茹. 拟南芥仅含START结构域蛋白质的生物学功能研究[J]. 农业生物技术学报, 2022, 30(9): 1649-1661.
LIU Xia, LI Xiao-Kai, CHANG Hong-Juan, LIU Tong-Tong, WANG Feng-Ru. Study on Biological Function of Proteins Containing Only START Domain in Arabidopsis thaliana. 农业生物技术学报, 2022, 30(9): 1649-1661.
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