Transcriptome Analysis of Mouse (Mus musculus) Kidney After Infection with Serotype A Pasteurella multocida
LIU Zhi-Yong, CHENG Yi-Wen, HUANG Hui-Xian, JIANG Jun-Ming, DU Li, MANCHU Ri-Ga, CHEN Si, WANG Feng-Yang, CHEN Qiao-Ling*
College of Animal Science and Technology, Hainan University/Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research of Hainan Province/Animal Genetic Engineering Key Laboratory of Haikou City, Haikou 570228, China
Abstract：Pasteurellosis is a zoonosis caused by Pasteurella multocida (Pm), which has a serious impact on the development of livestock economy. However, the specific pathogenic mechanism of Pm infection is still not fully understood. This study aimed to explore the immune changes induced by Pm infection in the kidney tissue and the infection mechanism of Pm. The mouse (Mus musculus) model of goat-derived Pm (HN02 strain) infection was established and its kidney samples were collected for transcriptome sequencing. Compared with the control group, there were 3 836 differentially expressed genes (DEGs) in Pm infection group, of which 1 741 DEGs were significantly up-regulated and 1 426 DEGs were significantly down-regulated; Gene Ontology (GO) function enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation results found that DEGs were mainly significantly enriched in signal transduction, energy metabolism and immune response, which mainly included transmembrane transporter activity, mitochondrion, tumor necrosis factor (TNF) signaling pathway, Human papillomavirus (HPV) infection, and mitogen-activated protein kinase (MAPK) signaling pathway in the process of Pm infecting mouse kidney tissue. After interaction analysis of immune related signaling pathways, some DEGs were found to participate in multiple different signaling pathways commonly, such as DEGs including v-rel avian reticuloendotheliosis viral oncogene homolog A (ReLA), nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA), tumor necrosis factor receptor superfamily, member 1A (TNFRSF1A), epidermal growth factor (EGF) and phosphoinositide-3-kinase, regulatory subunit 3 (PIK3R3). Then they were selected for qRT-PCR validation. The results of qRT-PCR showed that the expression trends of these genes were consistent with the sequencing results. This study preliminarily proved that kidney involved in a series of immune responses in the process of resisting Pm infection. The innate immunity of kidney tissue plays an important role in the process of resisting Pm infection, and this result provides data support for further research on the interaction mechanism between Pm and the host.
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