Cloning and Function Analysis of Root Development Associated Genes CsRDAs in Tea Plants (Camellia sinensis)
CHEN Rong1, LIU Hui1, FU Ping2, ZHAO De-Gang1, HUANG Xiao-Zhen2,1,*
1 The Key Laboratory of Plant Resources Conservation and Germplasm Innovationin Mountainous Region (Ministry of Education)/College of Life Sciences, Guizhou University, Guiyang 550025, China; 2 College of Tea Sciences, Guizhou University, Guiyang 550025, China
Abstract：Tea plants (Camellia sinensis), as an important commercial crop, are one of the popular and economic beverages worldwide. Root formation of tea plants is essential for higher yields and stable quality. To elucidate the programmed root developmental processes, the root development associated genes (CsRDAs) were isolated and cloned by reverse transcription-PCR (RT-PCR), which was based on the root transcriptome library of Guizhou landrace tea cultivars, called C. sinensis cv. Qiancha 1 (QC1) and C. sinensis cv. QianMei601 (QM601). They were named CsRDA1 (GenBank No. MW451597), CsRDA2 (GenBank No. MZ516824), CsRDA3 (GenBank No. MZ516825) and CsRDA4 (GenBank No. MZ516826), respectively. Phylogenetic and the domain analysis showed that the CsRDAs were evolutionally conservative and contained similar motifs. qPCR results showed that the 4 members of CsRDAs could specifically express in different tissues and developmental stages, which showed that their functions may be tissue specific and developmentally controlled. Meanwhile, transient expression of CsRDAs fusion protein in Nicotiana benthamiana leaves revealed that CsRDA1 and CsRDA2 mainly localized in the nucleus and cytoplasm, while CsRDA3 localized in the chloroplast and cytoplasm. Furthermore, the transgenic rice (Oryza sativa) plants which overexpressed CsRDA1 were successfully obtained by Agrobacterium tumefaciens-mediated callus transformation. Preliminary results showed that constitutive expression of CsRDA1 increased the number of roots in rice plants. These data suggested that CsRDA1 may participate in the regulation of the development of tea plant root system. It provides a theoretical reference for further elucidating the regulation network of tea plant radicle development.
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