Construction of Circular RNA-associated ceRNA Network in the Differentiation of Chicken (Gallus gallus) Preadipocytes
ZHANG Shan-Shan, LIU Ze-Jin, CHEN Lan, Wu Yu-Lin, DUAN Yan-Jun, DING Hao, ZHANG Tao*, ZHANG Gen-Xi, WANG Jin-Yu, XIE Kai-Zhou, DAI Guo-Jun
College of Animal Science and Technology, Yangzhou University/Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou 225009, China
Abstract：Circular RNA (circRNA) play important roles in the regulation of cell differentiation by acting as competing endogenous RNA (ceRNA). However, the regulatory mechanisms by which circRNAs and the circRNA-associated ceRNA network regulate chicken preadipocyte differentiation remain elusive. In this study, RNA-seq technology was used to analyze the expression of circRNAs, microRNAs (miRNAs) and mRNAs during the differentiation of chicken (Gallus gallus) preadipocytes. Bioinformatics methods were used to screen differentially expressed circRNAs, miRNAs and mRNAs, construct a circRNA-associated ceRNA regulatory network, and screen important circRNA-related ceRNA regulatory relationship pairs. A total of 4 904 circRNAs were identified in chicken preadipocytes. 143, 145 and 660 differentially expressed circRNAs, miRNAs and mRNAs were screened during the differentiation of chicken preadipocytes. Based on gene expression correlation analysis and target relationship prediction, a circRNA-associated ceRNA network was successfully constructed. Twelve candidate circRNA-miRNA-mRNA pairs were screened using qRT-PCR, which may play important regulatory roles in chicken preadipocyte differentiation. The present study lays a foundation for further analysis of the mechanism by which circRNAs regulating chicken fat deposition, and enriches the genetic regulation theory of chicken fat deposition.
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