向日葵MADS-box家族基因<em>HaTrans6</em>的克隆及表达分析

doi:10.3969/j.issn.1674-7968.2019.09.005

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摘要花发育进程对于植物的子代繁衍具有至关重要的意义,MADS-box家族基因在该进程中具有重要作用。为了探究MADS-box家族基因转录因子6 (transcription factor 6, Trans6)在向日葵(Helianthus annuus)花发育中的功能,本研究从向日葵中克隆得到HaTrans6基因(GenBank No. XM_022141206.1),并对其进行了系统分析。基因序列信息分析结果显示,HaTrans6具有典型的MADS-box和K-box保守结构域,HaTrans6为MADS-box候选基因。系统发育树分析结果显示,HaTrans6基因与拟南芥(Arabidopsis thaliana) Agamous-like 13 (AGL13)及AGL6基因具有较近的同源关系。基因表达模式分析结果显示,HaTrans6基因在向日葵花和籽粒中高表达;从花器官原基期到开花后5 d有较高的稳定表达,且在花器官原基期达到峰值。此外,HaTrans6基因在向日葵开花前5 d和开花当天的苞片、冠毛、花瓣、雄蕊、雌蕊和子房等花器官中均有表达,在子房中具有最高的表达丰度,其次是苞片。上述结果初步表明HaTrans6基因可能在向日葵花发育和早期果实发育进程中发挥调控作用,本研究为进一步探讨HaTrans6基因的功能提供了基础资料。

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	韦小英
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关键词 ：向日葵, HaTrans6基因, 基因表达, 花发育

Abstract：Flower development has vital significance for the reproduction of plant offspring, and the genes of MADS-box family play an important role in this process. In order to explore the function of the MADS-box gene transcription factor 6 (Trans6) in the flower development of sunflower (Helianthus annuus), HaTrans6 gene (GenBank No. XM_022141206.1) was cloned from sunflower and was analyzed systematically. The information analysis of gene sequence showed that HaTrans6 had typical MADS-box and K-box conserved domains, and HaTrans6 was a candidate member gene of MADS-box. Phylogenetic tree analysis showed that HaTrans6 gene had the closest homologous relationship with Agamous-like 13 gene (AGL13) and AGL6 gene of Arabidopsis thaliana. The expression pattern analysis indicated that HaTrans6 gene was highly expressed in flower and immature seed. The high and stable expression level was observed from floral organ primordial stage to 5 d after flowering, and reached its top level at floral organ primordial stage. In addition, it was shown that HaTrans6 gene was expressed in bract, crown, petal, stamen, pistil and ovary on 5 d before and the very day of flowering, and the highest expression abundance was found in the ovary, followed by bracts. The above results suggested that Hatrans6 gene might play an important role in regulating floral development and early fruit development of the sunflower. The present study provides basic data for further investigation about the function of Hatrans6 gene.

Key words： Helianthus annuus HaTrans6 gene Gene expression Floral development

收稿日期: 2019-03-27

ZTFLH:

S184

基金资助:国家自然科学基金(No. 31171587)、西华师范大学2018年博士科研启动专项(No. 18Q039)和西华师范大学英才基金(No. 463354)

通讯作者: zoujian@cwnu.edu.cn

引用本文:

韦小英, 何卓远, 雷豆, 苏周, 吴雨, 杨军, 邹建. 向日葵MADS-box家族基因HaTrans6的克隆及表达分析[J]. 农业生物技术学报, 2019, 27(9): 1560-1568.
WEI Xiao-Ying, HE Zhuo-Yuan, LEI Dou, SU Zhou, WU Yu, YANG Jun, ZOU Jian. Cloning and Expression Analysis of MADS-box Gene HaTrans6 in Sunflower (Helianthus annuus). 农业生物技术学报, 2019, 27(9): 1560-1568.

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http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2019.09.005 或 http://journal05.magtech.org.cn/Jwk_ny/CN/Y2019/V27/I9/1560

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