Identification and Expression Characterization Analysis of Trihelix GeneFamily in Mung Bean (Vigna radiata)
LI Meng-Jiao1, XING Zhan-Ning1,2, YU Qing1,2, LIU Fei1, XING Bao-Long3*, LIU Zhi-Ping1
1 High Latitude Crops Institute, Shanxi Agricultural University, Datong 037008, China; 2 College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, China; 3 Department of Social Service, Shanxi Agricultural University, Taiyuan 030031, China
Abstract：Trihelix transcription factor is a plant-specific transcription factor that plays an important role in plant seed formation, flower development, light regulation and stress resistance. In order to analyze Trihelix transcription factor role in the growth and development process and stress response, 38 Trihelix family members (VrGT) were identified in the genome of Mung bean (Vigna radiata) via bioinformatic analyses, which were unevenly distributed on 1~10 chromosome and classified into 5 sub-families. Members of the same sub-family shared the similar motifs types and quantities, and contained specific conserved motifs. Most of the VrGT genes contained 1~2 introns. Fragment duplication was the main driver of the expansion of the VrGT genes. There were 12 and 30 VrGT genes that were interspecific collinearity with Arabidopsis thaliana and soybean (Glycine max) genomes, respectively. Numerous of cis-acting elements related to plant growth and development, light responsiveness, hormones responsiveness and stress responsiveness were predicted in the promoter regions (-2000 bp) of the VrGT genes. Multiple transcription factor families, such as WRKY, MYB and bHLH, were coexpressed with VrGT genes. The relative expression patterns of 38 VrGT genes were different at different growth stages, in different tissues of plants and under different abiotic stresses, among which the expression level was the highest in germinated seeds and 4 genes had high expression levels during the growth and development process of mung bean. There were 3 genes that had a high response level in the early and late stages of drought stress, and 8 genes that had a significant regulatory effect under salinity- alkalinity stress. This study provides a reference for further understanding the biological function of VrGT family in mung bean.
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