Research Progress of CRISPR/Cas Gene Editing Technology in Herbicide-resistant Crop Breeding
TONG Chao-Yun1,*, HUANG Yu-Ting1,*, YANG Jing-Ru1, WANG Zhi-Rui1, LIU Xiao-Shuang1, LI Juan2,**, WEI Peng-Cheng1,**
1 College of Agronomy, Anhui Agricultural University, Hefei 230031, China; 2 Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China
Abstract：The creation and selection of superior new germplasm resistant to herbicides is a highly cost-effective and environmentally friendly method of weed control. CRISPR/Cas gene editing technology, characterized by simplicity, efficiency, and precision, holds tremendous potential for application in crop breeding. This study summarizes the research progress of CRISPR/Cas gene editing technology in herbicide-resistant crop breeding, with a focus on the types of crop herbicides, the mutant sites of resistance gene targets, and the development of CRISPR/Cas-mediated precision editing technology. It also provides a detailed introduction to the application of gene editing technology in the creation of superior new herbicide-resistant crop germplasm. Finally, by elaborating on the existing challenges and future development directions of CRISPR/Cas gene editing technology, this study provides a theoretical basis and technical support for the creation of new herbicide-resistant crop germplasm.
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