Abstract：With the advent of CRISPR/Cas system, plant genome editing has entered a new era of precise editing for any genes of interest. The CRISPR/Cas toolkits have been successfully tested on a wide variety of fruit tree species. According to the statistics of breeding research, researchers have edited about 45 fruit tree genes by CRISPR/Cas genome editing technology, which were mainly used to enhance disease resistance, prolong shelf-time, early flowering, tree dwarf and increase shoot branching. However, the fruit tree genome editing still relies mostly on Cas9-based indel mutation and Agrobacterium-mediated stable transformation. Transient transformation for transgene-free genome editing is preferred, but it typically has very low efficiency in fruit trees, substantially limiting its potential utility. In this review, the CRISPR/Cas system and its related technologies were summarized, and then we introduced the delivery methods of the CRISPR /Cas expression vector into plant genome and the current status of fruit tree genome editing practices using the CRISPR/Cas system, and discussed the problems impeding the efficient application of the CRISPR/Cas toolkits for fruit trees genome editing, as well as future prospects. This review provides reference for research on gene function and molecular breeding of fruit trees.
倪海枝, 王引, 王平, 程玉芳, 颜帮国, 陈方永. CRISPR/Cas 基因组编辑技术在果树育种中的应用[J]. 农业生物技术学报, 2023, 31(8): 1730-1746.
NI Hai-Zhi, WANG Yin, WANG Ping, CHENG Yu-Fang, YAN Bang-Guo, CHEN Fang-Yong. Application of CRISPR/Cas Genome Editing Technology in Fruit Trees Breeding. 农业生物技术学报, 2023, 31(8): 1730-1746.
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