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33
2025年5月15日 星期四
农业生物技术学报  2025, Vol. 33 Issue (5): 1063-1072    DOI: 10.3969/j.issn.1674-7968.2025.05.011
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
植物甾醇酯对小鼠骨骼肌发育的影响
冯晓华*, 谭伟豪*, 孙丽娟, 赵伟杰, 陈履双, 谢开来, 朱树庆, 冯霞洁, 孙中华, 吴鑫, 王丽娜**
华南农业大学 动物科学学院/广东省动物营养调控重点实验室,广州 510642
Effects of Phytosterol Esters on Mice (Mus musculus) Skeletal Muscle Development
FENG Xiao-Hua*, TAN Wei-Hao*, SUN Li-Juan, ZHAO Wei-Jie, CHEN Lyu-Shuang, XIE Kai-Lai, ZHU Shu-Qing, FENG Xia-Jie, SUN Zhong-Hua, WU Xin, WANG Li-Na**
Guangdong Provincial Key Laboratory of Animal Nutrition Control/College of Animal Science, South China Agricultural University, Guangzhou 510642, China
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摘要 植物甾醇酯(phytosterol esters, PE)是植物甾醇与脂肪酸酯化形成的活性物质,具有抗炎和调节免疫功能等特性。为探究PE对小鼠(Mus musculus)骨骼肌发育的影响,本研究选取3周龄小鼠随机分成对照组和实验组(即0.3% PE组),通过饲养实验及其体组成、肌肉力量和运动耐力等检测,探究PE对肌肉发育的影响;通过免疫荧光和qPCR检测肌纤维面积和类型以及生肌因子的变化,最后通过ELISA和Western blot方法分析白介素含量及其受体下游信号通路的变化。结果表明,1)饲粮添加0.3% PE可显著增加小鼠平均日采食量(P<0.05),且腓肠肌指数显著上调(P<0.05),皮下脂肪指数出现下调的趋势。2)饲粮添加0.3% PE可显著提升小鼠抓握力与举重分数(P<0.05),而跑步时间和跑步距离无显著变化。3)饲粮添加0.3% PE可显著增加腓肠肌和比目鱼肌的平均肌纤维横截面积(P<0.05),显著上调了生肌因子5 (myogenic factor 5, Myf5) mRNA水平(P<0.05),且肌分化因子(myogenic differentiation, MyoD)和肌细胞生成素(myogenin)蛋白水平也有上调的趋势。4)饲粮添加0.3% PE可显著升高血清中的白介素-11 (interlukin, IL-11)水平(P<0.05),Janus激酶2 (Janus kinase 2, JAK2)磷酸化水平有升高的趋势,信号转导因子和转录激活因3 (signal transducers and activators of transcription 3, STAT3)磷酸化水平显著升高(P<0.05)。综上所述,饲粮添加PE可以促进小鼠骨骼肌发育,增加肌纤维直径,提升肌肉功能,其促进骨骼肌发育的机制可能与IL-11及其下游JAK2/STAT3信号通路有关。本研究为PE在生产上促进肌肉发育提供理论依据。
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冯晓华
谭伟豪
孙丽娟
赵伟杰
陈履双
谢开来
朱树庆
冯霞洁
孙中华
吴鑫
王丽娜
关键词 植物甾醇酯肌肉发育肌纤维类型白细胞介素-11 (IL-11)JAK2/STAT3信号通路    
Abstract:Phytosterol esters (PE) are active substances formed by esterification of phytosterols with fatty acids. In order to investigate the effects of PE on skeletal muscle development in mice (Mus musculus), 3-week-old mice were randomly divided into control and experimental groups (i.e., the 0.3% PE group), and the effects of PE on muscle development were detected by feeding test, body composition, grip strength and weight lifting test, changes in myofibre area and type as well as myogenic factors were detected by immunofluorescence and qPCR, and finally, the interleukin content and its receptor downstream signaling pathway were analysed by ELISA and Western blot. The results showed that, 1) Dietary supplementation with 0.3% PE significantly increased the average daily intake of mice (P<0.05), and the gastrocnemius index was significantly up-regulated (P<0.05), while the subcutaneous fat index showed a down-regulation trend. 2) Dietary supplementation with 0.3% PE significantly improved the grip strength and weight lifting scores of mice (P<0.05), while there was no significant change in the running time and the distance of the run. 3) Dietary supplementation with 0.3% PE significantly increased the mean muscle fibre cross-sectional area of gastrocnemius and soleus muscles (P<0.05), significantly up-regulated myogenic factor 5 (Myf5) mRNA levels (P<0.05), and there was a tendency for up-regulation in the levels of myogenic differentiation (MyoD) and myogenin proteins as well. (4) Dietary supplementation of 0.3% PE significantly increased serum levels of interleukin-11 (IL-11)(P<0.05), Janus kinase 2 (JAK2) phosphorylation level had a tendency to increase, and signal transducers and activators of transcription 3 (STAT3) phosphorylation level was significantly increased (P<0.05). In conclusion, dietary addition of phytosterol esters could promote skeletal muscle development, increase muscle fibre diameter and enhance muscle function, which might be related to IL-11 and its downstream JAK2/STAT3 signaling pathway. This study provides a theoretical basis for phytosterol esters to promote muscle development in production.
Key wordsPhytosterol esters    Muscle development    Muscle fiber type    Interleukin-11 (IL-11)    JAK2/STAT3 signaling pathway
收稿日期: 2024-06-19     
中图分类号: S816
基金资助:国家自然科学基金面上项目(32072779); 国家重点研发计划(2022YFD1300401); 以农产品为单元的广东省现代农业产业技术体系创新团队建设项目(饲料)(2023KJ115)
通讯作者: **wanglina@scau.edu.cn   
作者简介: *同等贡献作者
引用本文:   
冯晓华, 谭伟豪, 孙丽娟, 赵伟杰, 陈履双, 谢开来, 朱树庆, 冯霞洁, 孙中华, 吴鑫, 王丽娜. 植物甾醇酯对小鼠骨骼肌发育的影响[J]. 农业生物技术学报, 2025, 33(5): 1063-1072.
FENG Xiao-Hua, TAN Wei-Hao, SUN Li-Juan, ZHAO Wei-Jie, CHEN Lyu-Shuang, XIE Kai-Lai, ZHU Shu-Qing, FENG Xia-Jie, SUN Zhong-Hua, WU Xin, WANG Li-Na. Effects of Phytosterol Esters on Mice (Mus musculus) Skeletal Muscle Development. 农业生物技术学报, 2025, 33(5): 1063-1072.
链接本文:  
https://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2025.05.011     或     https://journal05.magtech.org.cn/Jwk_ny/CN/Y2025/V33/I5/1063
 
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