Analysis of Mating Type Composition and Distribution of Setosphaeria turcica in Northern Maize Producting Areas of China
WANG Chao-Hong1, ZHANG Lu1, GUO Zheng-Yu2, YAN Xiao-Guang3, LIU Xin-Fang4, LIU Ning1, CAO Zhi-Yan1,*, DONG Jin-Gao1,*
1 College of Plant Protection/State Key Laboratory of North China Crop lmprovement and Regulation, Hebei Agricultural University, Baoding 071000, China; 2 Institute of Corn Research, Shanxi Agricultural University, Xinzhou 034000, China; 3 Institute of Millet Research, Shanxi Agricultural University, Changzhi 046011, China; 4 Corn Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110065, China
Abstract：Setosphaeria turcica can harm the leaves, leaf sheaths and bracts of maize (Zea mays), resulting in the decrease of maize yield and quality, thus causing serious economic losses. Northern corn leaf blight has become one of the most important fungal diseases on maize in China. There were 3 mating types (MAT) in S. turcica, namely A mating type containing only StMAT1-1 gene, a mating type containing only StMAT1-2 gene, and Aa mating type containing both genes simultaneously. In China, there were significant differences in the composition and distribution of mating types of S. turcica in China between years and regions, leading to frequent genetic variations of the pathogens. To clarify the composition and distribution of mating types of the pathogen in different years of maize production areas in northern China, in this study, mating type identification specific primers of S. turcica were used to determine the mating type of 365 isolates collected from 10 provinces (autonomous regions) of Shaanxi, Shanxi, Henan, Hebei, Gansu, Heilongjiang, Ningxia, Inner Mongolia, Liaoning and Jilin in China from 2018 to 2022. The results showed that a total of 61 isolates of type A, 224 isolates of type a, and 80 isolates of type Aa were detected from 365 isolates of S. turcica, with isolation frequencies of 16.71%, 61.37%, and 21.92%, respectively, indicating that a mating type was the main mating type in North China. There were little differences in the frequency of mating types in S. turcica populations between different years. The isolation frequencies of mating type a in 2018, 2019, 2020, 2021, and 2022 was 61.73%, 72.50%, 55.74%, 58.65%, and 63.29%, respectively. There were differences in the composition of mating types of S. turcica in different regions. No A mating type isolate was isolated in Heilongjiang Province, while the distribution of A, a, and Aa mating types, were found in the remaining 9 provinces (autonomous regions). However, there were differences in the distribution frequency of different mating type isolates among regions. The frequency of a mating type isolates was the highest in Heilongjiang Province, at 87.50%, and the frequency of A mating type isolates was the highest in Shaanxi Province, at 26.67%, the frequency of Aa mating type isolates was the highest in Inner Mongolia and Jilin provinces, both at 33.33%, indicating that there was diversity in mating types among S. turcica populations. This study further clarified the composition and distribution of mating types of S. turcica in northern China, which provides reference for the analysis of pathogen variation trends and effective control.
王超红, 张璐, 郭正宇, 阎晓光, 刘欣芳, 刘宁, 曹志艳, 董金皋. 中国北方玉米产区玉米大斑病菌交配型组成及其分布分析[J]. 农业生物技术学报, 2024, 32(5): 1141-1149.
WANG Chao-Hong, ZHANG Lu, GUO Zheng-Yu, YAN Xiao-Guang, LIU Xin-Fang, LIU Ning, CAO Zhi-Yan, DONG Jin-Gao. Analysis of Mating Type Composition and Distribution of Setosphaeria turcica in Northern Maize Producting Areas of China. 农业生物技术学报, 2024, 32(5): 1141-1149.
[1] 陈小丹. 2022. 玉米大斑病的发生及防治[J]. 现代农村科技, (08): 42-43.(Chen X D. 2022. The occurrence and prevention of Setosphaeria turcica[J]. Modern Rural Technology, (08): 42-43.) [2] 戴冬青, 王绍新, 刘宁, 等. 2019. 玉米大斑病菌交配型组成鉴定及其有性生殖条件优化[J]. 植物保护学报, 46(03): 634-641. (Dai D Q, Wang S X, Liu N, et al.2019. Identification of mating type and optimization of sexual reproduction conditions of northern leaf blight fungus Setosphaeria turcica[J]. Journal of Plant Protection, 46(03): 634-641.) [3] 郭建国, 杨凤珍, 杜蕙, 等. 2018. 甘肃省玉米大斑病菌生理小种结构组成与交配型测定[J]. 植物保护, 44(06): 85-90. (Guo J G, Yang F Z, Du H, et al.2018. Structural composition of physiological races and mating types of Setosphaeria turcica in Gansu[J]. Plant Protection, 44(06): 85-90.) [4] 郭丽媛, 贾慧, 曹志艳, 等. 2013. 玉米大斑病菌有性杂交后代的交配型与寄生适合度分化[J]. 中国农业科学, 46(19): 4058-4065. (Cuo L Y, Jia H, Cao Z Y, et al.2013. Analysis on mating type and parasitic fitness diversity in sexual hybridization offsprings of Setosphaeria turcica[J]. Scientia Agricultura Sinica, 46(19): 4058-4065.) [5] 刘微微, 郭丽婕, 贾慧, 等. 2014. 玉米大斑病菌交配型基因克隆及生物信息学分析[J]. 华北农学报, 29(05): 10-15. (Liu W W, Cuo L J, Jia H, et al.2014. Cloning and bioinformatics analysis of MAT genes in Setosphaeria turcica[J]. Acta Agriculturae Boreali-sinica, 29(05): 10-15.) [6] 孟庆江. 2015. 玉米大斑病菌黑色素合成调控基因StMR1转录活性及调控机制分析[D]. 硕士学位论文, 河北农业大学, 导师: 董金皋, 曹志艳, pp. 13-14. (Meng Q J.2015. The transcriptional activity and regulation mechanism of regulated gene StMR1 in melanin biosynthesis pathway of Setosphaeria turcica[D]. Thesis for M.S., Hebei Agricultural University, Suppervisor: Dong J G, Cao Z Y, pp. 13-14.) [7] 马周杰, 何世道, 庞欣宇, 等. 2021. 东北地区玉米大斑病菌交配型组成及遗传多样性分析[J]. 沈阳农业大学学报, 52(04): 402-408. (Ma Z J, He S D, Pang X Y, et al.2021. Analysis of mating type composition and genetic diversity of Setosphaeria turcica in Northeast China[J]. Journal of Shenyang Agricultural University, 52(04): 402-408.) [8] 王利智, 康志钰, 吴景芝, 等. 2011. 云南省玉米大斑病菌生理小种的鉴定及交配型分析[J]. 华中农业大学学报, 30(02): 187-192. (Wang L Z, Kang Z Y, Wu J Z, et al.2011. Identification of physiological races and analysis of mating type of Setosphaeria turcica in Yunnan Province[J]. Journal of Huazhong Agricultural University, 30(02): 187-192.) [9] Bi Y, Hu J, Cui X, et al.2014. Sexual reproduction increases the possibility that Phytophthora capsici will develop resistance to dimethomorph in China[J]. Plant Pathology, 63(6): 1365-1373. [10] Cui L, Zhao L, Wang B, et al.2022. Genetic diversity, population structure and mating type distribution of Setosphaeria turcica on corn in Midwestern China[J]. Journal of Fungi, 8(11): 1165-1175. [11] Dai Y, Gan L, Lan C, et al.2021. Genetic differentiation and mixed reproductive strategies in the northern corn leaf blight pathogen Setosphaeria turcica from sweet corn in Fujian province, China[J]. Frontiers in Microbiology, 12: 1343-1356. [12] Heitman J.2010. Evolution of eukaryotic microbial pathogens via covert sexual reproduction[J]. Cell Host Microbe, 8(1): 86-99. [13] Human M P, Barnes I, Craven M, et al.2016. Lack of population structure and mixed reproduction modes in Exserohilum turcicum from South Africa[J]. Phytopathology, 106(11): 1386-1392. [14] Jindal K K, Tenuta A U, Woldemariam T, et al.2019. Occurrence and distribution of physiological races of Exserohilum turcicum in Ontario, Canada[J]. Plant Disease, 103(7): 1450-1457. [15] Li Y G, Jiang W Y, Zhang Q F, et al.2019. Population structure and genetic diversity of Setosphaeria turcica from corn in Heilongjiang province, China[J]. Journal of Applied Microbiology, 127(6): 1814-1823. [16] Ma Z, Hui H, Huang Y, et al.2022. Evaluation of maize hybrids for identifying resistance to northern corn leaf blight in Northeast China[J]. Plant Disease, 106(3): 1003-1008. [17] Perkins J M, Pedersen W L.1987. Disease development and yield losses associated with leaf northern blight on corn[J]. Plant Disease, 71(10): 940-943. [18] Sun S, Coelho M A, David-Palma M, et al.2019. The evolution of sexual reproduction and the mating-type locus: Links to pathogenesis of Cryptococcus human pathogenic fungi[J]. Annual Review of Genetics, 53(1): 417-444. [19] Thatcher S, Leonard A, Lauer M, et al.2023. The northern corn leaf blight resistance gene Ht1 encodes an nucleotide-binding, leucine-rich repeat immune receptor[J]. Molecular Plant Pathology, 24(7): 758-767. [20] Weems J D, Bradley C A.2018. Exserohilum turcicum race population distribution in the North Central United States[J]. Plant Disease, 102(2): 292-299. [21] Wu D, Turgeon B G.2013. Setosphaeria rostrata: Insights from the sequenced genome of Setosphaeria turcica[J]. Fungal Genetics and Biology, 61: 158-163. [22] Zwonitzer J C, Bubeck D M, Bhattramakki D, et al.2009. Use of selection with recurrent backcrossing and QTL mapping to identify loci contributing to southern leaf blight resistance in a highly resistant maize line[J]. Theoretical and Applied Genetics, 118(5): 911-925.
