Isolation and Identification of A Myxobacterial Strain E10 and Optimization of Fermentation Conditions for Its Metabolites Against Phytophthora infestans
LIU Ya-Ping1*, ZHANG Yu2*, DING Yi-Xiu2, LI Jun-Da2, SHANG Shao-Jie2, ZHAO Xiao-Jing2, LIU Tao2, HOU Ke-Xin2, GAO Yan-Dong2, WANG Ning-Bin2, LIU Hui-Rong2**
1 Yakeshi City Senfeng Potato Industry Limited Liability Company, Hulun Buir 021008, China; 2 College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China
Abstract：Myxobacteria are a group of higher prokaryotes with abundant and diverse metabolites. Phytophthora infestans is a pathogen of potato (Solanum tuberosum) late blight, which causes 10%~30% loss of potato industry in China every year. Myxobacteria have significant antagonistic activity against P. infestans, so it is of great research value to screen the active substances with antagonistic activity against P. infestans from the metabolites of myxobacteria. In this study, myxobacteria were isolated from soil samples in Ordos area and their antagonistic activity against P. infestans was detected. Then, the strains were identified by morphological and molecular biological methods, and the optimal fermentation conditions for the strains to produce the active substances against P. infestans and the effect of macroporous resin adsorption on the activity of myxobacterial metabolites were investigated by orthogonal experimental design. The results showed that the isolated strain E10 had a strong antagonistic activity against P. infestans, the diameter of the inhibition zone was 26 mm, the colony was circular and expanding, the edge was wavy, the fruiting body was yellow or orange-red, ridge-like protrusions, and coral-like branches.The strain belonged to Corallococcus exiguus. MD1 medium was the optimal fermentation medium for the strain, and 32 ℃, 0 concentration of NaCl and fermentation for 11 d were the optimal fermentation conditions for the strain. The addition of macroporous resin could effectively adsorb the active substances against P. infestans in the fermentation broth and increase the synthesis of these products, thus increasing their antagonistic ability. This study is of great significance for the future research and development of biopesticides against potato late blight.
刘雅萍, 张玉, 丁一秀, 李俊达, 尚少杰, 赵晓静, 刘涛, 候可心, 高艳东, 王宁斌, 刘惠荣. 粘细菌菌株E10的分离鉴定及其拮抗致病疫霉活性物质发酵条件的优化[J]. 农业生物技术学报, 2024, 32(12): 2870-2881.
LIU Ya-Ping, ZHANG Yu, DING Yi-Xiu, LI Jun-Da, SHANG Shao-Jie, ZHAO Xiao-Jing, LIU Tao, HOU Ke-Xin, GAO Yan-Dong, WANG Ning-Bin, LIU Hui-Rong. Isolation and Identification of A Myxobacterial Strain E10 and Optimization of Fermentation Conditions for Its Metabolites Against Phytophthora infestans. 农业生物技术学报, 2024, 32(12): 2870-2881.
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