Genetic Diversity Analysis of Dwarf Tomato Germplasm Resources Based on Agronomic Traits and InDel Markers
KAN Yun-Xia1, SHI Hai-Lin1, ZHANG Dan-Dan1,YOU Xi1, YOU Yuan-Yuan1, SHU Jin-Shuai4, WANG Shuai1,2,3*, MAO Xiu-Jie1,2,3
1 College of Horticultural Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China; 2 Hebei Key Laboratory of Characteristic Horticultural Germplasm Mining and Innovative Utilization, Qinhuangdao 066004, China; 3 Hebei Higher Institute Application Technology Research and Development Center of Horticultural Plant Biological Breeding, Qinhuangdao 066004, China; 4 Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Abstract：Dwarf tomato (Solanum lycopersicum) has both ornamental and edible values, and its abundant germplasm resources are the basis for germplasm innovating and varieties breeding. In order to study the genetic diversity of germplasm resources and screen more diverse types of germplasm resource materials for parental selection and variety breeding, 25 traits of 45 dwarf tomato germplasm resources from domestic and foreign were investigated, and genetic diversity analysis as well as cluster analysis were carried out; 33 pairs of InDel primers were used to PCR amplification to analyze their genetic diversity. The results showed that the genetic diversity index of 25 traits ranged from 0.26 to 2.03, the diversity index of the 7 mass traits ranged from 0.26 to 1.31, with the average value of 0.92, and the coefficients of variation of the 18 quantitative traits were distributed from 8.51% to 32.51%, with the average value of 19.01%. The mean number of alleles effective for InDel marker detection was 1.64, and the polymorphic information content (PIC) ranged from 0.18 to 0.43, with the average value of 0.31. Both agronomic trait cluster analysis and InDel marker cluster analysis divided 45 materials into 3 categories, and the agronomic traits of most germplasm were consistent with the clustering results of InDel markers. The results showed that 45 dwarf tomato germplasm resources had rich genetic diversity, which could provide reference basis for new varieties breeding.
阚云霞, 师海林, 张丹丹, 游茜, 尤园园, 舒金帅, 王帅, 毛秀杰. 基于农艺性状和 InDel 标记的矮生番茄种质资源遗传多样性分析[J]. 农业生物技术学报, 2023, 31(6): 1122-1132.
KAN Yun-Xia, SHI Hai-Lin, ZHANG Dan-Dan1,YOU Xi, YOU Yuan-Yuan, SHU Jin-Shuai, WANG Shuai, MAO Xiu-Jie. Genetic Diversity Analysis of Dwarf Tomato Germplasm Resources Based on Agronomic Traits and InDel Markers. 农业生物技术学报, 2023, 31(6): 1122-1132.
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