The Function on Dimorphic Transition of the Uegpa3 Gene in Ustilago esculenta
YU Jin-Meng*, ZHANG Ya-Fen*, GE Qian-Wen, HU Ying-Li, GAO Li-Dan, XIA Wen-Qiang, YE Zi-Hong**
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine/College of Life Sciences, China Jiliang University, Hangzhou 310018, China
Abstract：Ustilago esculenta can infect Zizania latifolia to induce edible fleshy stem, which called Jiaobai in China. It was found that U. esculenta was a dimorphic fungus, there is transformation between yeast type and mycelium type, and this dimorphic transition was related to its pathogenicity. When the lipopeptide pheromone encoded by mfa (mating factor) gene and pheromone receptor encoded by the pra (pheromone receptor) gene recognize each other, they activate cyclic adenosine monophosphate-protein kinase A (cAMP-PKA) signal transduction pathway and mitogen-activated protein kinase cascade (MAPK) signaling pathways, which make the fusion of 2 yeast-type compatible strains and form hyphae, then have pathogenicity. In this study, the gene Uegpa3 (Ustilago esculenta guanine uncleotide-binding protein subunit alpha-3)(GenBank No. ALS87611.1), encoding the α subunit of G protein, was cloned based on the whole genome analysis of U. esculenta. The cDNA length of Uegpa3 is 1 065 bp, without introns, encoding 354 amino acids, with a typical Gα domain. The expression patterns analysis showed that the expression of Uegpa3 was up-regulated during conjunction tube formation. Uegpa3 deletion mutant was constructed based on PEG (polyethylene glycol) mediated protoplast transformation and it was found that the Uegpa3 deletion strain could not fuse to form mycelium. Furthermore, the ability of conjunction tube was lost in Uegpa3 mutant during mating and the expression of pheromone synthesis gene was significantly inhibited. The above results demonstrated that Uegpa3 affected the conjugation tube formation in the dimorphic transition in U. esculenta through regulating phenome recognition. This study preliminarily explored the function of the Uegpa3, a gene encoding one of the G protein α subunits in U. esculenta, and discussed its role in dimorphic transition, which provide basic material for the research on the interaction mechanism between the U. esculenta and Z. latifolia.
于金梦, 张雅芬, 葛倩雯, 胡映莉, 高丽丹, 夏文强, 叶子弘. Uegpa3基因在菰黑粉菌二型态转换中的作用[J]. 农业生物技术学报, 2020, 28(5): 892-902.
YU Jin-Meng, ZHANG Ya-Fen, GE Qian-Wen, HU Ying-Li, GAO Li-Dan, XIA Wen-Qiang, YE Zi-Hong. The Function on Dimorphic Transition of the Uegpa3 Gene in Ustilago esculenta. 农业生物技术学报, 2020, 28(5): 892-902.
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