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2025年4月6日 星期日
  2018, Vol. 26 Issue (6): 920-930    
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
菰ZlWRKY72转录因子克隆及其在茎部膨大发育中的表达分析
周美琪1,李帅1,叶子弘2,张雅芬2,俞晓平2,赵金兰1,崔海峰2
1. 中国计量大学
2. 中国计量学院
Cloning of ZlWRKY72 Transcription Factor and Its Expression Analysis During the Development of Swollen Stem of Zizania latifolia
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摘要 摘 要 菰黑粉菌(Ustilago esculenta)侵染菰(Zizania latifolia)植株后能够诱导其茎部发生形态转变,但其肉质茎膨大发育的调节机制尚不清楚。本研究克隆获得菰WRKY转录因子72(WRKY transcription factor 72, ZlWRKY72)(GenBank登录号:MG365889)的全长序列,其基因序列全长为2 831 bp,含有1个内含子。cDNA 全长为1 085 bp,ORF编码框为738 bp,编码245 个氨基酸,具有1 个典型WRKYGQK结构域和1个Cys2.His2锌指结构,进化树分析结果表明,ZlWRKY72与粳稻(Oryza sativa subsp. japonica)OsWRKY72同源性最高。通过qRT-PCR技术检测ZlWRKY72基因在其茎部膨大发育中的表达,表明ZlWRKY72在菰植株组织生长旺盛的部位表达量较高,分蘖期叶片表达量显著高于茎部(P<0.05),但孕茭起始后茎部表达量显著高于叶片(P<0.05)。茎部ZlWRKY72表达变化与菰黑粉菌菌丝侵染增殖相关,孕茭起始后正常茭白茎部表达量高于灰茭(P<0.05),并在随后的膨大发育过程中表达量逐渐降低,差异显著(P<0.05)。雄茭茎部表达量高于正常茭白,茭白茎部菰黑粉菌菌丝大量侵染增殖后ZlWRKY72表达下调。本研究初步阐明了ZlWRKY72在菰肉质茎膨大发育中的表达响应,探讨了其在菰黑粉菌侵染诱导的茎部膨大发育中的调节作用,为菰孕茭膨大发育机制研究提供了理论依据。
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周美琪
李帅
叶子弘
张雅芬
俞晓平
赵金兰
崔海峰
关键词 菰WRKY转录因子72(ZlWRKY72)茎部发育菰黑粉菌表达模式    
Abstract:Abstract The growth and development of stem could be induced and changed by the infection of Ustilago esculenta in Zizania latifolia, but the regulatory of swollen stem is still unclear. In this research, Z. latifolia WRKY transcription factor 72 (ZlWRKY72) (GenBank number: MG365889) was cloned with full DNA length of 2 831 bp with one intron. The full length of its cDNA was 1 085 bp with a 738 bp of ORF coding frame encoding 245 amino acids, the typical structure domain of WRKYGQK and a zinc finger structure of Cys2.His2 were found in ZlWRKY72. The phylogenetic analysis showed that the ZlWRKY72 has the highest homology with Oryza sativa subsp. japonica OsWRKY72. Expression analysis showed that the higher expressions of ZlWRKY72 during the development of swollen stem of Z. latifolia, liking the higher expression in leaves than stems at tillering stage seedling (P<0.05), while the higher expression in stems than leaves at stage of initiation swollen using qRT-PCR (P<0.05). The expression of ZlWRKY72 in stem was related to the successfully infection and reproduction of U. esculenta. After the initiation of swollen stem, the higher expression of ZlWRKY72 were detected in the stem of Jiaobai than grey Jiaobai (P<0.05), which was reduced significantly during the development of swollen stem in Jiaobai (P<0.05). The expression of ZlWRKY72 were higher in male Z. latifolia than Jiaobai during the initiation of swollen. It was found that the reduction expression of ZlWRKY72 occurred following with the increasing hypha reproduction of U. esculenta in stem. The expression responses and regulation of ZlWRKY72 are investigated and discussed during the development of swollen stem in Z. latifolia, which might be used in the research of swollen stem. It provides a theoretical basis for studying the mechanism of swelling development of Zizania latifolia.
Key wordsZizania latifolia    WRKY transcription factor 72 (ZlWRKY72)    Stem development    Ustilago esculenta    Expression pattern
收稿日期: 2017-12-08      出版日期: 2018-05-21
基金资助:国家重点研发计划项目;国家自然科学基金项目;国家自然科学基金项目
通讯作者: 崔海峰     E-mail: hfcui@cjlu.edu.cn
引用本文:   
周美琪 李帅 叶子弘 张雅芬 俞晓平 赵金兰 崔海峰. 菰ZlWRKY72转录因子克隆及其在茎部膨大发育中的表达分析[J]. , 2018, 26(6): 920-930.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2018/V26/I6/920
 
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