根瘤农杆菌介导玉米<em>ZmHDZIV13</em>和<em>ZmHDZIV14</em>基因的遗传转化及干旱抗性分析

doi:10.3969/j.issn.1674-7968.2019.04.003

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摘要同源异型域-亮氨酸拉链(homeodomain-leucine zipper, HD-Zip)转录因子在植物的生长发育、形态建成及抵抗各种逆境胁迫中起着十分重要的调控作用。本研究构建了植物表达载体pCAMBIA3300-Ubi-ZmHDZIV13/14-bar,利用根瘤农杆菌(Agrobacterium tumefaciens)茎尖转化法将玉米ZmHDZIV13和ZmHDZIV14基因转入玉米自交系'郑58',以Basta除草剂筛选阳性植株,以PCR及Southern blot方法对转基因玉米进行检测;在干旱胁迫下对T₂代转基因玉米和非转基因玉米株系进行耐旱性分析。结果表明,正常处理下,转基因株系与非转基因株系均能正常生长,根和叶中丙二醛(malondialdehyde, MDA)、H₂O₂、脯氨酸(proline, Pro)、可溶性糖(soluble sugar, SS)含量、过氧化物酶(peroxidase, POD)、过氧化氢酶(catalase, CAT)及超氧化物歧化酶(superoxide dismutase, SOD)活性无显著差异;但在干旱胁迫条件下,转ZmHDZIV13和ZmHDZIV14基因玉米株系根和叶中的MDA和H₂O₂含量极显著降低(P<0.01),Pro、SS含量、POD、CAT及SOD活性极显著增高(P<0.01)。上述结果表明,导入ZmHDZIV13和ZmHDZIV14基因在一定程度上提高了玉米植株对干旱胁迫的耐受能力。本研究为深入探讨ZmHDZIV13和ZmHDZIV14基因功能和创制转基因抗旱材料提供了基础资料。

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	闫慧萍
	赵小强
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	方鹏
	任斌
	庄泽龙
	高巧红
	曾文静

关键词 ：玉米, ZmHDZIV13基因, ZmHDZIV14基因, 农杆菌茎尖原位转化法, 耐旱性

Abstract：HD-Zip (homeodomain-leucine zipper) is one of the important transcription factors, which acts important roles in regulating plant growth, development, morphogenesis, and resistance to various abiotic stress. In the present study, the plant expression vector pCAMBIA3300-Ubi-ZmHDZIV13/14-bar was succeeded constructed, the ZmHDZIV13 and ZmHDZIV14 genes were transferred into maize inbred line 'Zheng 58' by Agrobacterium tumefaciens-mediated stem dip transformation, and the transgenic maize lines were screened by Basta herbicide, and were detected by PCR and Southern blot method. After that, T₂ transgenic and non-transgenic maize lines were used to analyze the drought tolerance. Results showed that the non-transgenic/transgenic maize lines grew normal growth without stress, and there was no significant difference in the contents of the malondialdehyde (MDA), H₂O₂, proline (Pro), soluble sugar (SS) contents, and the activity of peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD) in roots and leaves between both maize lines; however, under drought stress, the contents of MDA and H₂O₂ decreased significantly(P<0.01), whereas, the contents of Pro and SS, and activity of POD, CAT, and SOD increased significantly(P<0.01) in roots and leaves of ZmHDZIV13 and ZmHDZIV14 transgenic maize lines. These results indicated that ZmHDZIV13 and ZmHDZIV14 genes could improve the drought tolerance in maize plants. Therefore, this study could provide the basis data for the further study of ZmHDZIV13 and ZmHDZIV14 genes and creation of transgenic drought-tolerant materials.

Key words： Maize ZmHDZIV13 gene ZmHDZIV14 gene Agrobacterium tumefaciens-stem dip transformation Drought resistance

收稿日期: 2018-08-16

ZTFLH:

S330

基金资助:国家重点研发计划(No. 2018YFD0100203-4)、甘肃省重点研发计划(No. 18YF1NA071)、甘肃省自然科学基金(No. 18JR3RA189)、中科院“西部之光”项目(No. 20180504)、兰州市科技计划项目(No. 2018-1-103)和甘肃省重大科技专项(No. 17ZD2NA016)

通讯作者: pengyunlingpyl@163.com

引用本文:

闫慧萍, 赵小强, 彭云玲, 方鹏, 任斌, 庄泽龙, 高巧红, 曾文静. 根瘤农杆菌介导玉米ZmHDZIV13和ZmHDZIV14基因的遗传转化及干旱抗性分析[J]. 农业生物技术学报, 2019, 27(4): 593-605.
YAN Hui-Ping, ZHAO Xiao-Qiang, PENG Yun-Ling, FANG Peng, REN Bin, ZHUANG Ze-Long, GAO Qiao-Hong, ZENG Wen-Jing. Agrobacterium tumefaciens-mediated Genetic Transformation and Drought Resistance Analysis of ZmHDZIV13 and ZmHDZIV14 in Maize (Zea mays). 农业生物技术学报, 2019, 27(4): 593-605.

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http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2019.04.003 或 http://journal05.magtech.org.cn/Jwk_ny/CN/Y2019/V27/I4/593

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