Abstract：HD-Zip (homeodomain-leucine zipper) is one of the important transcription factors, which acts important roles in regulating plant growth, development, morphogenesis, and resistance to various abiotic stress. In the present study, the plant expression vector pCAMBIA3300-Ubi-ZmHDZIV13/14-bar was succeeded constructed, the ZmHDZIV13 and ZmHDZIV14 genes were transferred into maize inbred line 'Zheng 58' by Agrobacterium tumefaciens-mediated stem dip transformation, and the transgenic maize lines were screened by Basta herbicide, and were detected by PCR and Southern blot method. After that, T2 transgenic and non-transgenic maize lines were used to analyze the drought tolerance. Results showed that the non-transgenic/transgenic maize lines grew normal growth without stress, and there was no significant difference in the contents of the malondialdehyde (MDA), H2O2, proline (Pro), soluble sugar (SS) contents, and the activity of peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD) in roots and leaves between both maize lines; however, under drought stress, the contents of MDA and H2O2 decreased significantly(P<0.01), whereas, the contents of Pro and SS, and activity of POD, CAT, and SOD increased significantly(P<0.01) in roots and leaves of ZmHDZIV13 and ZmHDZIV14 transgenic maize lines. These results indicated that ZmHDZIV13 and ZmHDZIV14 genes could improve the drought tolerance in maize plants. Therefore, this study could provide the basis data for the further study of ZmHDZIV13 and ZmHDZIV14 genes and creation of transgenic drought-tolerant materials.
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