Identification of Differentially Expressed Transcription Factors in Sesame (Sesamum indicum) in Response to High Temperature and Expression Characteristics Analysis of SiMYB4 Gene
WANG Dong-Yong1,2, ZHANG Peng-Yu1,2, LI Feng1,2, TIAN Yuan1,2, RONG Ya-Si1, LU Hai-Ling1, WAN Fu-Qiang3, GAO Tong-Mei1,2,*
1 Sesame Research Center/Henan Key Laboratory of Specific Oilseed Crops Genomics, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 2 The Shennong Laboratory, Zhengzhou 450002, China; 3 Pingyu County Agriculture and Rural Affairs Bureau, Zhumadian 463400, China
Abstract：High temperature is an important factor that restricts the healthy and sustainable development of sesame (Sesamum indicum) production. The aim of this study is to reveal the molecular mechanism and mining key transcription factors in response to high temperature stress of sesame, and analyze the expression characteristics of candidate genes under different stresses. In this study, the leaves of heat-tolerant sesame variety 'Zhengtaizhi No.3' and heat-sensitive sesame variety 'Shandong White Sesame' were collected after 10 d of high temperature treatment for transcriptome sequencing. The results showed that 954 transcription factors were identified in 2 sesame varieties under high temperature treatment, and 255 transcription factors were differentially expressed, accounting for 26.73% of the total. The most differentially expressed transcription factor families were bHLH (basic helix-loop-helix), AP2/ERF (ethylene responsive factor), NAC (NAM, ATAF1/2, CUC1/2), MYB (avian myeloblastosis viral oncogenehomolog) and C2H2 families. Compared with CK, 191 and 206 differentially expressed transcription factors were detected in 'Shandong white sesame' and 'Zhengtaizhi No.3' under high temperature treatment, respectively, among which 50 and 65 transcription factors were specifically expressed in 'Shandong White Sesame' and 'Zhengtaizhi No.3', respectively. The Venn diagram showed that a total of 141 transcription factors were differentially expressed in 2 varieties, of which 38 transcription factors were up-regulated and 103 transcription factors were down-regulated. In addition, 36 thermotolerant transcription factors were identified, among which MYB family had the largest number of transcription factors. Six key candidate genes in response to high temperature stress, such as SiMYB4, SibZIP41 and SiHsfB2b were screened by co-expression network analysis. The promoter region of the thermotolerant SiMYB4 contains multiple hormone and stress response elements. qRT-PCR results showed that SiMYB4 was induced to express under high temperature, drought, high salinity and ABA treatments. The expression of SiMYB4 reached the maximum at 48 h of high temperature and ABA treatment, which were 10.28 times and 10.80 times that of 0 h respectively, and the differences were significant. Under drought, high salt and MeJA treatments, the expression of SiMYB4 gene increased first and then decreased. This study provides theoretical basis and candidate genes for further exploring the molecular mechanism heat resistance in sesame.
王东勇, 张鹏钰, 李丰, 田媛, 戎亚思, 芦海灵, 万富强, 高桐梅. 芝麻高温响应差异表达转录因子鉴定及SiMYB4基因的表达特性分析[J]. 农业生物技术学报, 2025, 33(7): 1451-1462.
WANG Dong-Yong, ZHANG Peng-Yu, LI Feng, TIAN Yuan, RONG Ya-Si, LU Hai-Ling, WAN Fu-Qiang, GAO Tong-Mei. Identification of Differentially Expressed Transcription Factors in Sesame (Sesamum indicum) in Response to High Temperature and Expression Characteristics Analysis of SiMYB4 Gene. 农业生物技术学报, 2025, 33(7): 1451-1462.
[1] 蒋玲玉. 2019. 玉米响应高温胁迫的转录组测序及关键调控基因的鉴定与分析[D]. 硕士学位论文, 安徽师范大学, 导师: 钱叶雄, pp. 22-70. (Jiang L Y.2019. Transcriptome sequencing and identification and analysis of key regulatory genes in response to heat stress in maize[D]. Thesis for M.S., Anhui Normal University, Supervisor: Qian Y X, pp. 22-70.) [2] 庞强强, 孙晓东, 周曼, 等. 2024. 菜心叶片响应高温胁迫的转录组分析及转录因子筛选[J]. 分子植物育种, 22(08): 2473-2487. (Pang Q Q, Sun X D, Zhou M, et al.2024. Transcriptome analysis of Chinese flowering cabbage leaves and transcription factor screening under heat stress[J]. Molecular Plant Breeding, 22(08): 2473-2487.) [3] 王辉, 董永梅, 郭伟锋, 等. 2023. 高温胁迫下陆地棉转录组差异性分析[J]. 新疆农业科学, 60(11):2618-2626. (Wang H, Dong Y M, Guo W F, et al.2023. Analysis of transcriptome differences in upland cotton under high temperature stress[J]. Xinjiang Agricultural Sciences, 60(11): 2618-2626.) [4] 卫双玲, 高桐梅, 吴寅, 等. 2015. 高温胁迫对芝麻光合特性及产量的影响[J]. 西南农业学报, 28(5): 1977-1981. (Wei S L, Gao T M, Wu Y, et al.2015. Effects of high temperature stress on photosynthetic characteristic and grain yield in sesame (Sesamum indicum L.)[J]. Southwest China Journal of Agricultural Sciences, 28(5): 1977-1981.) [5] Bokszczanin K.2013. Perspectives on deciphering mechanisms underlying plant heat stress response and thermotolerance[J]. Frontier in Plant Science, 4: 315. [6] Chaudhary S, Devi P, Bhardwaj A J, et al.2020. Identification and characterization of contrasting genotypes/cultivars for developing heat tolerance in agricultural crops: Current status and prospects[J]. Frontier in Plant Science, 11: 587264. [7] Cheng Z Y, Luan Y T, Meng J S, et al.2021. WRKY transcription factor response to high-temperature stress[J]. Plants, 10(10): 2211. [8] El-Kereamy A, Bi Y M, Ranathunge K, et al.2012. The rice R2R3-MYB transcription factor OsMYB55 is involved in the tolerance to high temperature and modulates amino acid metabolism[J]. PLOS ONE, 7(12): e52030. [9] Guo Y P, Zhou H F, Zhang L C.2006. Photosynthetic characteristics and protective mechanisms against photooxidation during high temperature stress in two citrus species[J]. Scientia Horticulturae, 108(3): 260-267. [10] Hasanuzzaman M, Nahar K, Alam M M, et al.2013. Physiological, biochemical, and molecular mechanisms of heat stress tolerance in plants[J]. International Journal of Molecular Science, 14(5): 9643-9684. [11] He Y A, Li W, Lv J, et al.2012. Ectopic expression of a wheat MYB transcription factor gene, TaMYB73, improves salinity stress tolerance in Arabidopsis thaliana[J]. Journal of Experimental Botany, 63(3): 1511-1522. [12] Kraus K, Hnilickova H, Pecka J, et al.2022 The effect of the application of stimulants on the photosynthetic apparatus and the yield of winter wheat[J]. Agronomy, 12(1): 78. [13] Lescot M, Dehais P, Thijs G, et al.2002. PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences[J]. Nucleic Acids Research, 30(1): 325-327. [14] Li T, Xu X, Li Y, et al.2024. Comparative transcriptome analysis reveals differential transcription in heat-susceptible and heat-tolerant pepper (Capsicum annum L.) cultivars under heat stress[J]. Journal of Plant Biology, 58(6): 411-424. [15] Li W, Cui X, Meng Z, et al.2012. Transcriptional regulation of Arabidopsis MIR168a and ARGONAUTE1 homeostasis in abscisic acid and abiotic stress responses[J]. Plant Physiology, 158(3): 1279-1292. [16] Love M I, Huber W, Anders S.2014. Moderated estimation of foldchange and dispersion for RNA-seq data with DESeq2[J]. Genome Biology, 15(12): 550-571. [17] Ohama N, Sato H, Shinozaki K, et al.2017. Transcriptional regulatory network of plant heat stress response[J]. Trends in Plant Science, 22(1): 53-65. [18] Qin Q Q, Zhao Y J, Zhang J J, et al.2022. A maize heat shock factor ZmHsf11 negatively regulates heat stress tolerance in transgenic plants[J]. BMC Plant Biology, 22(1): 406. [19] Rotundo J L, Tang T, Messina C D.2019. Response of maize photosynthesis to high temperature: Implications for modeling the impact of global warming[J]. Plant Physiology and Biochemistry, 141: 202-205. [20] Scharf K D, Berberich T, Ebersberger I, et al.2012. The plant heat stress transcription factor (Hsf) family: Structure, function and evolution[J]. Biochimica et Biophysical Acta, 1819(2): 104-119. [21] Schmittgen T D, Livak K J.2008. Analyzing real-time PCR data by the comparative CT method[J]. Nature Protocol, 3(6): 1101-1108. [22] Su X Y, Gao T M, Zhang P Y, et al.2022. Comparative physiological and transcriptomic analysis of sesame cultivars with different tolerance responses to heat stress[J]. Physiology and Molecular Biology of Plants, 28(5): 1131-1146. [23] Trapnell C R A, Goff L, Pertea G, et al.2012. Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks[J]. Nature Protocol, 7: 562-578. [24] Wang C P, Zhou Y Z, Yang X, et al.2022. The heat stress transcription factor LlHsfA4 enhanced basic thermotolerance through regulating ROS metabolism in lilies (Lilium longiflorum)[J]. International Journal of Molecular Science, 23(1): 572. [25] Wang C T, Ru J N, Liu Y W, et al.2018. Maize WRKY transcription factor ZmWRKY106 confers drought and heat tolerance in transgenic plants[J]. International Journal of Molecular Science, 19(10): 3046. [26] Wang J Y, Chen C, Wu C Z, et al.2023. SlMYB41 positively regulates tomato thermotolerance by activating the expression of SlHSP90.3[J]. Plant Physiology and Biochemistry, 204: 108106. [27] Wang K H, Liu Y R, Tian J L, et al.2017. Transcriptional profiling and identification of heat-responsive genes in perennial ryegrass by RNA-sequencing[J]. Frontier in Plant Science, 8: 1032. [28] Wei L B, Miao H M, Zhao R H, et al.2013. Identification and testing of reference genes for sesame gene expression analysis by quantitative real-time PCR[J]. Planta, 237(3): 873-889. [29] Wei W, Yang Y Y, Lakshmanan P, et al.2023. Proteasomal degradation of MaMYB60 mediated by the E3 ligase MaBAH1 causes high temperature-induced repression of chlorophyll catabolism and green ripening in banana[J]. Plant Cell, 35(5): 1408-1428. [30] Xie Z L, Nolan T M, Jiang H, et al.2019. AP2/ERF transcription factor regulatory networks in hormone and abiotic stress responses in Arabidopsis[J]. Frontiers in Plant Science, 10: 228. [31] Xing G L, Li J Y, Li W L, et al.2021. AP2/ERF and R2R3-MYB family transcription factors: Potential associations between temperature stress and lipid metabolism in Auxenochlorella protothecoides[J]. Biotechnology for Biofuels and Bioproducts, 14(1): 22. [32] Zha Q, Xi X J, He Y N, et al.2022. Interaction of VvbZIP60s and VvHSP83 in response to high-temperature stress in grapes[J]. Gene, 810: 146053. [33] Zha Q, Yin X J, Xi X J, et al.2023. Heterologous VvDREB2c expression improves heat tolerance in Arabidopsis by inducing photoprotective responses[J]. International Journal of Molecular Science, 24(6): 5989. [34] Zhang X M, Cheng Z H, Yao W J, et al.2021. Functional characterization of PsnNAC036 under salinity and high temperature stresses[J]. International Journal of Molecular Science, 22(5): 2656. [35] Zhang X W, Li J P, Liu A L, et al.2012. Expression profile in rice panicle: Insights into heat response mechanism at reproductive stage[J]. PLOS ONE, 7(11): e49652. [36] Zhao C, Liu B, Piao S L, et al.2017. Temperature increase reduces global yields of major crops in four independent estimates[J]. Proceedings of the National Academy of Sciences of the USA, 114(35): 9326-9331. [37] Zheng Y J, Yang Z Q, Xu C, et al.2020. The interactive effects of daytime high temperature and humidity on growth and endogenous hormone concentration of tomato seedlings[J]. HortScience, 55(10): 1575-1583.
