Evaluation of Ovarian Development and Comparative Transcription Study of Channel Catfish (Ictalurus punctatus) Pseudofemale
XU Si-Qi1, ZHANG Shi-Yong1,2,*, WANG Ming-Hua1,2, LIU Hong-Yan1, ZHONG Li-Qiang1,2, CHEN Xiao-Hui1,2,*
1 Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, China;
2 The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing 210014, China
Abstract：The cultivation of XY type pseudo female fish is a necessary step in obtaining faster growing all male strains of channel catfish (Ictalurus punctatus). In order to investigate the ovarian development at the period of adult and transcriptomic expression during sex differentiation after XY female channel catfish was induced by 17β-estradiol. The ovarian development and oocyte development of channel catfish were compared and analyzed by histological and anatomical methods with positive genetic sex identification, and the gonad development of XY pseudo-female channel catfish at the 3-year-old (500~2000 g) was evaluated by statistical gonad index. Transcriptomic sequencing was performed on XY pseudo-female channel catfish and normal female channel catfish at the age of 60 d after hatching. The gonad index of the 3-year-old XY female channel catfish was significantly different, the ovary of the large size XY females and the normal females was larger, with long sacs, densely filled with blood vessels and full of yolk. Oocytes in vitellogenic stage were mainly accompanied by cortical follicular stage and a little number of primary growth oocytes. Transcriptome sequencing revealed 11 288 differentially expressed genes between the ovaries of 60 d after hatching XY type pseudo female fish and normal female fish, enriched in multiple immune related biological processes and signaling pathways. According to GO enrichment and KEGG signaling pathway, among the top 30 GO enriched pathways, the female gamete generation pathway was screened, in which 32 genes showed differential expression, with down regulation of progesterone receptor (pgr) gene and androgen receptor (ar) gene expression. The research provides reproductive biology techniques and theoretical basis for sex control breeding of channel catfish.
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