Genetic Diversity Analysis of Cauliflower (Brassica oleracea var. botrytis) Breeding Germplasm Based on SSR Markers
SONG Xiao-Yu1,2, HAN Shuo1, GAN De-Fang2, SONG Shun-Hua1, LIU Fan1, GUO Ning1,*, MENG Shu-Chun1,*
1 State Key Laboratory of Vegetable Biobreeding/National Engineering Research Center for Vegetables/ Inspection and Test Center of Vegetable Seed Quality of Ministry of Agriculture and Rural Affairs/Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; 2 Horticulture College, Anhui Agricultural University, Anhui 230036, China
Abstract：Cauliflower (Brassica oleracea var. botrytis), originating from Europe, has a relatively narrow genetic diversity in China's germplasm resources, urgently requiring studies on germplasm innovation and evaluation techniques. The creation of double haploids (DH) using microspore culture not only shortens the breeding cycle but is also a crucial means for germplasm innovation. Understanding and evaluating the genetic diversity of cauliflower microspore progeny is a key issue to be resolved for the application of DH technology in germplasm innovation and breeding. In this study, 29 pairs of SSR primers with clear and stable band patterns and good polymorphism were selected, with PIC range of 0.29 to 0.57 and average value of 0.42. Using these 29 primers, the marker genotyping study was conducted on a cauliflower breeding germplasm population consisting of 66 DH lines and 18 advanced inbred lines, totaling 84 materials. The results showed that the genetic distances of the materials mainly ranged from 0.3 to 0.6, with an average of 0.56; the genetic similarity coefficients ranged from 0.50 to 0.97, with an average of 0.57. At a genetic similarity coefficient of 0.55, the materials were divided into 4 groups. Through the analysis of the relationships of DH materials, it was found that high genetic diversity could be obtained in the DH progenies of F1 hybrid, confirming that DH breeding could serve as an important tool for germplasm innovation in cauliflower. This study provides theoretical and technical support for the evaluation and utilization of genetic diversity in cauliflower.
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SONG Xiao-Yu, HAN Shuo, GAN De-Fang, SONG Shun-Hua, LIU Fan, GUO Ning, MENG Shu-Chun. Genetic Diversity Analysis of Cauliflower (Brassica oleracea var. botrytis) Breeding Germplasm Based on SSR Markers. 农业生物技术学报, 2024, 32(9): 1982-1995.
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