Preliminary Study on ZmMYC7 Transcription Factor Regulating of Disease Resistance Related Functions in Maize (Zea mays)
WANG Yong-Gui1,*, LI Wei1,*, CUI Ting-Ru2, ZHANG Fu-Yuan1, YANG Wen-Hao1, XU Dang-Fei3, XING Ji-Hong1, CAO Hong-Zhe1,**, DONG Jin-Gao1,**
1 State Key Laboratory of North China Crop Improvement and Regulation/Hebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding 071000, China; 2 Baoding Meteorological Service, Baoding 071000, China; 3 Zhangjiakou Forestry Station, Zhangjiakou 075000, China
Abstract：Maize (Zea mays) is one of the most important food crops in the world. Maize diseases caused by pathogens directly affect maize production and lead to huge economic losses. Transcription factor MYC2 plays an important role in the field of plant disease resistance. ZmMYC7 (Zm00001d030028), ortholog of Arabidopsis thaliana AtMYC2, was previously obtained in the previous work. In order to explore the function of transcription factor ZmMYC7 in regulating maize disease resistance, the sensitivity of ZmMYC7 mutant to 4 maize fungal diseases was detected, and the effect of ZmMYC7 mutation on the expression level of pathogenesis-related protein gene ZmPRs was detected by qRT-PCR. The results showed that ZmMYC7 had the highest similarity (90%) with Sorghum bicolor SbMYC2. ZmMYC7 was highly expressed in leaves and anthers of maize. The expression of ZmMYC7 was up-regulated by UV irradiation and Fusarium graminearum infection. In ZmMYC7 mutants, the sensitivity of maize to leaf and stem diseases was significantly increased, and the expression level of disease resistance related gene ZmPRs was significantly down-regulated (P<0.01). The above results showed that ZmMYC7 played an important role in maize resistance to pathogen disease. The present study provides basic materials for further analysis of the molecular mechanism of ZmMYC7 regulating maize disease resistance.
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