Cloning and Expression Analysis of the Zl-CRK5 Gene During the Swelling and Development of Stem in Zizania latifolia
ZHENG Yu-Jie, ZHOU Ya-Ping, MA Ying-Ran, WANG Jing, TANG Jin-Tian, XIA Wen-Qiang, ZHANG Ya-Fen, YE Zi-Hong, CUI Hai-Feng*
School of Life Sciences, China Jiliang University/Key Laboratory of Microbiological Metrology, Measurement & Bio-product Quality Security, State Administration for Market Regulation, Hangzhou 310018, China
Abstract：The swollen stem was induced by the infection of Ustilago esculenta in Zizania latifolia, following with the inhibition of polar growth of plants and the down-regulation of expression of auxin (IAA) polar transport related genes. In this research, the calcium-dependent protein kinase (CDPK) related kinase 5 (CRK5) gene of Z. latifolia was cloned with the full-length gDNA sequence of 4 224 bp, encoding 453 amino acids and containing the typical STKc_CAMK conformal domain of CRK kinase family. It was closely related to Oryza brachyantha. qPCR analysis showed that Zl-CRK5 was highly expressed in stem and leaf sheath of Z. latifolia (P<0.05), but lower in root and leaf (P<0.05). The expression of Zl-CRK5 in stems was significantly down-regulated (P<0.05) after 7 d infection of U. esculenta in plantlets of Z. latifolia. It was significantly down-regulated during the initial swolling stem of normal Z. latifolia (P<0.05), and contained lower expression level following the subsequent swollen and development (P<0.05), which was similar to the reported gene of Zl-PIN1b in Z. latifolia. Further analysis showed that the Zl-CRK5 gene and IAA polar transport-related genes PIN (PIN-formed) of Zl-PIN1b and Zl-PIN1c showed a coordinated expression relationship in the stems of Z. latifolia after treatment with IAA polar transport-inhibitor of N-1-Naphthylphthalamic (NPA). It was speculated that Zl-CRK5 might be involved in the regulation of polar growth of stems by activating the expression of IAA polar transport-related genes Zl-PIN1b and Zl-PIN1c. Further research would help to reveal the regulatory mechanism of stem expansion induced by infection of U. esculenta.
郑宇捷, 周雅萍, 马莹然, 王晶, 汤近天, 夏文强, 张雅芬, 叶子弘, 崔海峰. Zl-CRK5基因克隆及其在菰茎部膨大发育中的表达分析[J]. 农业生物技术学报, 2025, 33(5): 989-998.
ZHENG Yu-Jie, ZHOU Ya-Ping, MA Ying-Ran, WANG Jing, TANG Jin-Tian, XIA Wen-Qiang, ZHANG Ya-Fen, YE Zi-Hong, CUI Hai-Feng. Cloning and Expression Analysis of the Zl-CRK5 Gene During the Swelling and Development of Stem in Zizania latifolia. 农业生物技术学报, 2025, 33(5): 989-998.
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