Effects of Wheat Soybean Meal Diet Supplemented with ε-polylysine Hydrochloride on Laying Performance, Egg Quality and Liver Lipid Metabolism of Laying Hens (Gallus gallus domesticus)
LIU Meng-Qi, SHI Ming-Hua, WU Jian-Qing, FAN Jin-Yun, WANG Han, YANG Song-Bai, ZHAO A-Yong*, ZHOU Xiao-Long*
College of Animal Science and Technology•College of Veterinary Medicine, Zhejiang A & F University/Zhejiang Key Laboratory of Applied Technology Research on Green Ecological and healthy breeding of livestock and poultry, Hangzhou 311300, China
Abstract：China's annual soybean production is insufficient to meet the demands of production and daily life, resulting in a heavy reliance on imports. Internationally, the price of soybeans continues to soar. However, substituting wheat for corn can lead to a reduction in soybean meal usage. ε-Poly-L-lysine hydrochloride (ε-PLH) is a natural preservative and antimicrobial agent commonly employed in the food industry and other related fields. This study aimed to investigate the effects of adding ε-PLH to wheat-soybean meal feed on the production performance, egg quality, and hepatic lipid metabolism of laying hens (Gallus gallus domesticus). The experiment involved a total of 120 Hy-line brown laying hens at 21 weeks of age, which were randomly divided into 4 groups. Each group comprised 5 replicates, with 6 chickens per replicate. The groups were designated as follows: Control group, wheat meal group, wheat meal+0.01% ε-PLH group, and wheat meal+0.05% ε-PLH group. The hens were fed with the respective diets for 3 weeks during the adaptation period, followed by a formal experimental period of 12 weeks. The results indicated that there were no significant differences in laying rate and feed to egg ratio among the control group and the three treatment groups. However, the average egg weight of the wheat meal+0.01% ε-PLH group and the wheat meal+0.05% ε-PLH group was significantly higher than that of the control group (P<0.05). The egg shape index, albumen height, and haugh unit of the wheat meal group, wheat meal+0.01% ε-PLH group, and wheat meal+0.05% ε-PLH group were significantly higher than those of the control group (P<0.05). Additionally, the eggshell thickness of the wheat meal+0.01% ε-PLH group was significantly higher than that of the control group and the wheat meal group (P<0.05). Wheat meal+0.01% ε-PLH group could significantly up-regulate the expression of lipid metabolism related genes such as peroxisome proliferator-activated receptor gamma (PPARG), fatty acid desaturase 2 (FADS2) and fatty acid binding protein 1 (FABP1)(P<0.05). Thus, it could be inferred that substituting wheat for corn and supplementing with ε-PLH can improve the production performance and egg quality of laying hens, alleviate hepatic lipid deposition. This study provides theoretical and practical support for the application of ε-PLH in poultry farming and offers alternative solutions for national policies aiming to reduce the use of corn and soybean meal.
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