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| Effect of CIDEa Interference on Lipid Synthesis of Mammary Epithelial Cells in Dairy Goats (Capra hircus) |
| LI Jun1,2, SUN Yu-Ting3, GUO Zhen-Nan1, HAN Hao-Yuan1, ZHAO Jin-Yan1, XU Qiu-Liang1, NIU Hui, QUAN Kai1,* |
1 College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China;
2 International Joint Laboratory of Ruminant Nutrition and Feed Resources Development in Henan Province, Zhengzhou 450046, China;
3 Shaanxi Institute of Zoology, Xi'an 710032, China |
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Abstract Cell death inducing DNA fragmentation factor 45 like effector a (CIDEa) is a kind of protein closely related to lipid metabolism. It is involved in the regulation of lipolysis, the growth and fusion of lipid droplets and the maturation of very low density lipoprotein. In order to determine the biological function of CIDEa in dairy goat mammary gland, the mRNA level of CIDEa in mammary gland was detected using qPCR during lactation and dry period. Then, siRNA mediated interference technique was used to study the effect of CIDEa on lipid synthesis and the expression of related genes. The results showed that the expression of CIDEa mRNA in mammary gland was higher in lactation than that in dry period (P＜0.05). The siRNAs targeting
CIDEa gene were transfected into dairy goat mammary epithelial cells, and the mRNA level of CIDEa was
inhibited by 60%~88% (P＜0.01). The accumulation of lipid droplets and the content of triglyceride in cells
were inhibited after interfering CIDEa expression. In addition, interfering CIDEa expression significantly upregulated
the expression of hormone sensitive lipase (HSL) and fatty acid synthase (FASN) (P＜0.05), and also
promoted the expression of sterol regulatory element binding protein 1 (SREBP1) (P＜0.05). Furthermore, the
expression of fatty acid binding protein 3 (FABP3) and diacylglycerol transferase gene 1 (DGAT1) (P＜0.05)
were inhibited when interfering CIDEa gene expression. The results showed that CIDEa promoted the
synthesis of lipid droplets in dairy goat mammary epithelial cells, which provides a theoretical basis for further
study the molecular mechanism of CIDEa gene regulating milk lipid synthesis in dairy goat.
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Received: 31 July 2021
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Corresponding Authors:
*quankai1115@163.com
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