Expression of MSTN Gene and Its Association Analysis with Meat Quality in Tibetan Sheep (Ovis aries)
ZHANG Jun-Xia1,2, LEI Xiu-Cun2, HE Na2, CUI Ya-Jie2, LYU Cai-Ling2, LI Ming-Ming2, LANG Xia1*, WANG Cai-Lian1*
1 Institute of Animal & Pasture Science and Green Agriculture/Key Laboratory for Sheep, Goat, and Cattle Germplasm and Straw Feed in Gansu Province, Gansu Academy of Agricultural Science, Lanzhou 730070, China; 2 College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China
Abstract As a muscle growth inhibitor, myostain (MSTN) is a member of TGF- β superfamily. MSTN mutation will cause excessive muscle growth and a "double muscle" phenotype. The gene is of great significance for improving muscle yield and lean meat rate of animals. The current study aimed to analyze the correlation between MSTN gene and meat production traits of Tibetan sheep (Ovis aries), six 6-month-old Tibetan sheep with similar body weight and no disease were randomly selected in this study. After slaughtering, slaughter performance, meat quality and meat nutritional components of Tibetan sheep were determined. The mRNA expression levels of MSTN gene in brachial triceps, quadriceps femoris, longissimus dorsi and semitendinosus of Tibetan sheep were detected by qPCR, and the correlation between the expression level of MSTN gene and meat production traits was analyzed. The results showed that all slaughter performance indexes were no significant difference between ram and ewe. The slaughter rates of ram and ewe were 51.32% and 52.01%, respectively, as well as the carcass weights were 22.08 and 20.03 kg, respectively. The results of meat quality analysis showed that there was no significant difference between male and female sheep except shear force (P<0.05). There were no significant differences in the contents of crude protein, crude fat, crude ash and water between ram and ewe. The protein contents were 23.14% and 22.17% for ram and ewe, respectively, and the crude fat contents of ram and ewe were 2.46% and 2.87%, respectively. MSTN gene was expressed in all muscle tissues of Tibetan sheep, but the differences of the expression levels in all muscle tissues were not significant. By analyzing the correlation between the expression level of MSTN gene and each index, the mRNA expression level of MSTN gene in arm triceps of Tibetan sheep was significantly positively correlated with the live weight before slaughter (P<0.05, 1.000); The expression level in longissimus dorsi muscle was significantly positively correlated with the redness value (P<0.05, 0.831), and it was extremely significantly negatively correlated with cooked meat rate (P<0.01, -0.958); The expression level in quadriceps femoris muscle was significantly negative correlated with cooked meat rate (P<0.01,-1.000); There was no significant correlation between MSTN gene expression in semitendinosus muscle and meat production indexes. Expression of MSTN gene was correlated with meat producing traits of Tibetan sheep. This study provides theoretical basis for the study of the regulation mechanism of MSTN gene on meat quality of Tibetan sheep.
ZHANG Jun-Xia,LEI Xiu-Cun,HE Na, et al. Expression of MSTN Gene and Its Association Analysis with Meat Quality in Tibetan Sheep (Ovis aries)[J]. 农业生物技术学报, 2022, 30(5): 926-934.
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