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| The Impacts and Mechanism of Streptococcus agalactiae on Behavior of GIFT Tilapia (Oreochromis niloticus) |
| YI Meng-Meng, WANG Miao, LI Zhong-Hui, ZHANG De-Feng, LIU Zhi-Gang, Gao Feng-Ying, LU Mai-Xin* |
| Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture/Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, China |
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Abstract Streptococcus agalactiae infection may cause abnormal behavior of tilapia (Oreochromis niloticus) and seriously endangers the development of the tilapia industry.To investigate the impacts of S.agalactiae on behavior of GIFT tilapia and its mechanism, the qRT-PCR and immunofluorescence were used to examine the concentration of S.agalactiae in the brain and intestine, the behavior changes, as well as the expression of 5-hydroxytryptamine (5-HT) in the cerebellum, intestine and stomach of tilapia infected with by intraperitoneal injection.The results showed that the copies of S.agalactiae in the brain and intestinal tissues of tilapia increased sharply from the 6th and 0 h after pathogen infection respectively, and reached the maximum at 18th h (61.2 copies /mg in the brain and 1 800.5 copies/ mg in the intestine).Furthermore, fish showed abnormal behavior from the 12th h after pathogen challenge, and the abnormal behavior continued until the end of the experiment or the death of the fish.In addition, 5-HT positive reaction was found in the cerebellum, intestine and stomach of tilapia before and after S. agalactiae infection.While the positive reaction of 5-HT in cerebellum and intestine showed a weakening trend with the prolongation of experiment, and the fluorescence intensity of cerebellum and intestine were 9.1 and 6.9 at 18th h, respectively.But the 5-HT positive reaction in stomach increased first and then decreased with experiment went on, reaching a minimum of 3.1 at the end of the experiment.Therefore, it could be speculated that after infection of S.agalactia, tilapia induces abnormal behavior of tilapia by regulating 5-HT in the central nervous system and the peripheral digestive system.The current study researched the abnormal behavior of tilapia after S.agalactiae infection from the perspective of 5-HT, which could provide a new insight into the mechanism of impulsive behavior of fish infected with pathogen.
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Received: 23 November 2018
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Corresponding Authors:
mx-lu@163.com
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