Study on Variation of Anti-streptococcosis in 4 Hybrid Offspring of Oreochromis niloticus ♀× O. aureus ♂
ZHU Jing-Lin1, 2, ZOU Zhi-Ying1, LI Da-Yu1, XIAO Wei1, YANG Hong1, *, XUE Liang-Yi2, *
1 Key Laboratory of Freshwater Fisheries and GermPlasm Resources Utilization, Ministry of Agriculture Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; 2 College of Marine Science, Ningbo University, Ningbo 315211, China
Abstract Disease outbreak from Streptococcus agalactiae infection has become the most serious bacterial disease for tilapia (Oreochromis spp.) aquaculture in China, while effective prevention and control methods have not yet been established. It is an important approach to breeding the most S. agalactiae -resistant hybrid tilapia for better control and prevention S. agalactiae in tilapia. 4 family groups of hybrid tilapia F1 with different parents were got. They were O. niloticus Egypt strain ♀×O. aureus Xia'ao strain ♂ (NE ♀×AX ♂, EX), O. niloticus Xianghu strain ♀×O. aureus Xia'ao strain ♂(NX ♀×AX ♂, XX), O. niloticus Xianghu strain ♀×O. aureus Egypt strain ♂ (NX ♀×AE ♂, XE), O. niloticus Egypt strain ♀×O. aureus Egypt strain ♂ (NE ♀×AE ♂, EE). The offspring were strengthened rearing for 100 d in the ponds and taken back to the laboratory. All fish were acclimated for 14 d in the laboratory then artificially infected by S. agalactiae intraperitoneally. Fish were cultured on 33±1 ℃. The relative survival rate and serum biochemical parameters of 4 hybrid tilapia infected by S. agalactiae were investigated. The relative survival rate was recorded at different times post infection; Blood samples were respectively collected and analyzed. The relative survival rate at 7 d after infection was as follows: EX hybrid tilapia (50%) > EE hybrid tilapia (37.5%) > XE hybrid tilapia (33.3%)>XX hybrid tilapia (20.8%). Alanine transaminase (ALT) of EX hybrid tilapia showed no significantly difference (P>0.05) before and post injection, while that of other 3 species was significantly higher after infection(P<0.05);The serum electrolytes of 4 hybrid tilapia showed significantly difference after infection by S. agalactiae (P<0.05), but Na+, Ca2+ and Cl- of EX hybrid tilapia almost returned to control level at 3 d after infection. Globulin (GLO) of 4 hybrid tilapia increased statistically at 3 d after infection, and peaked at 7 d, while the albumin/globulin (A/G) index of 4 hybrid tilapia declined significantly at the time (P<0.05); Lactic dehydrogenase (LDH) of all 4 hybrid tilapia increased significantly on the progress of disease (P<0.05), and peaked with approximately 1.81-fold, 6.1-fold, 2.42-fold and 3.77-fold when compared with control samples prior to infection, respectively. Superoxide dismutase (SOD) activity of EE hybrid tilapia was significantly lower than that of the other species prior to infection, but SOD activity of EX hybrid tilapia was the lowest at 1 d and 7 d after infection. The triglyceride (TG) of 4 hybrid tilapia decreased significantly at 3 d after infection by S. agalactiae (P<0.05), while the total cholesterol (TC) of EX, XX and XE decreased significantly at 3 d after infection (P<0.05). Biochemical indexes had shown the 4 hybrid tilapia manifested liver and kidney damage, muscle injury and lipid metabolism after infection, while EX hybrid tilapia diserved less liver damage and muscle injury. And EX and EE hybrid tilapia showed less injury on metabolic activity. These results indicated that EX hybrid tilapia was the most resistant to S. agalactiae. In this study, the potential effects of the different tilapia orthogonal combinations were evaluated from the perspective of anti-streptococcosis. The expected results provide theoretical basis and potentially effective for genetic improvement of tilapia S. agalactiae -resistant breeding.
ZHU Jing-Lin,ZOU Zhi-Ying,LI Da-Yu, et al. Study on Variation of Anti-streptococcosis in 4 Hybrid Offspring of Oreochromis niloticus ♀× O. aureus ♂[J]. 农业生物技术学报, 2019, 27(1): 127-138.
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