|
|
|
| Gene Characterization of Ayu (Plecoglossus altivelis) IGF-1 and Its Functional Regulation on Monocytes/Macrophages |
| XU De-Zhi, LU Xin-Jiang*, LU Jian-Fei, CHEN Jiong |
| School of Marine Sciences, Ningbo University, Ningbo 315211, China |
|
|
|
|
Abstract Insulin-like growth factor-1 (IGF-1) is an important endocrine hormone which regulates cell growth and differentiation, and participates in the regulation of immune system.To investigate the mechanisms of IGF-1 in regulating fish immune response to pathogens and explore the regulation mechanism of IGF-1 on fish monocytes/macrophages (MO/MΦ), the present study got Plecoglossus altivelis PaIGF-1 cDNA sequence (GenBank No.MK005188) from transcriptome data.The sequence had 929 bp in length, contained an ORF which encoded a protein of 171 amino acids with the molecular weight of 18.86 kD.The amino acid sequence of PaIGF-1 was relatively conservative, sequence comparison showed that PaIGF-1 shared 77% amino acid sequence identity with Atlantic salmon (Salmo salar).Analysis of qRT-PCR showed that the expression of PaIGF-1 was in a high level in liver, trunk kidney, head kidney and intestine.After infected with Vibrio anguillarum, the expression of PaIGF-1 increased at 8 h in spleen, and increased at 24 h in head kidney, and there was no change in gill and liver.Furthermore, prokaryotic expression system was used to induce the expression and purification of PaIGF-1 recombinant protein (rPaIGF-1), and the mononuclear macrophages were incubated with rPaIGF-1.It was found that rPaIGF-1 increased the phagocytic activity of ayu MO/MΦ and had no effect on bacterial killing.Additionally, the qRT-PCR analysis showed that rPaIGF-1 increased the expression of interleukin-10 (IL-10) and transforming growth factor-β (TGF-β), suppressed the expression of IL-1β and tumor necrosis factor-α (TNF-α) in ayu MO/MФ infected with V.anguillarum.Finally, mononuclear macrophages PaCXCR (C-X-C chemokine receptor) 3.1+ and PaCXCR3.2+ were incubated with rPaIGF-1.It was found that rPaIGF-1 promoted the phosphorylation of PaSTAT3 in PaCXCR3.2+ but had no effect on the phosphorylation levels of PaSTAT1 and PaSTAT3 in PaCXCR3.1+.Inhibition of PaSTAT3 expression by specific siRNA could significantly down-regulate the expression of IL-10 and TGF-β in PaCXCR3.2+ treated with rPaIGF-1 which indicated that rPaIGF-1 might inhibit the inflammatory response of ayu MO/MΦ through activating PaSTAT3.The above research provides a theoretical basis to study PaIGF-1 function and related mechanism in regulating fish immune response to pathogens.
|
|
Received: 19 October 2018
|
|
|
|
Corresponding Authors:
lxj711043@163.com
|
|
|
|
[1] 华益民, 林浩然.2001.草鱼IGF-Ⅰ cDNA的克隆和在原核生物中的表达[J].动物学报, 47(3): 274-279.
(Hua Y M, Lin H R.2001.Cloning and expression in prokaryote of grass carp IGF-Ⅰ cDNA[J].Acta Zoologica Sinica, 47(3): 274-279.)
[2] 李长红, 陈炯, 史雨红, 等.2009.宁海地区香鱼弧菌病病原菌鉴定[J].微生物学报, 49(7): 931-937.
(Li C Y, Chen J, Shi Y H, et al.2009.Identification of pathogenic bacteria of Vibrio in ayu in Ninghai area[J].Acta Microbiologica Sinica, 49(7): 931-937.)
[3] 邹晓岚, 叶茸茸, 王洋, 等.2018.胰岛素样生长因子-1及其受体在肿瘤中的研究进展[J].重庆医学, 47(18): 2474-2479.
(Zou X L, Ye R R, Wang W, et al.2018.Progress in research of insulin-like growth factor-1 and its receptor in tumors[J].Chongqing Medicine, 47(18): 2474-2479.)
[4] Akdis M, Aab A, Altunbulakli C, et al.2016.Interleukins (from IL-1 to IL-38), interferons, transforming growth factor β, and TNF-α: Receptors, functions, and roles in diseases[J].Journal of Allergy and Clinical Immunology, 138(4): 984-1010.
[5] Anne-Constance F, Oliver F, Bernd K, et al.2016.Endocrine and local IGF-I in the bony fish immune System[J].Biology, 5(1): 1-13.
[6] Baer C, Squadrito M L, Laoui D, et al.2016.Suppression of microRNA activity amplifies IFN-γ-induced macrophage activation and promotes anti-tumour immunity[J].Nature Cell Biology, 18(7): 790-802.
[7] Bo Y X, Song X H, Wu K, et al.2015.Characterization of interleukin-1β as a proinflammatory cytokine in grass carp (Ctenopharyngodon idella)[J].Fish & Shellfish Immunology, 46(2): 584-595.
[8] Caldarone E M, Maclean S A, Beckman B R.2016.Evaluation of nucleic acids and plasma IGF1 levels for estimating short-term responses of postsmolt Atlantic salmon (Salmo salar) to food availability[J].Fishery Bulletin, 114(3): 288-301.
[9] Cao Q P, Duguay S J, Plisetskaya E, et al.1989.Nucleotide sequence and growth hormone-regulated expression of salmon insulin-like growth factor I mRNA[J].Molecular Endocrinology, 3(12): 2005-2010.
[10] Chen M H, Lin G, Gong H, et al.2001.The characterization of prepro-insulin-like growth factor-1 Ea-2 expression and insulin-like growth factor-1 genes (devoid 81 bp) in the zebrafish (Danio rerio)[J].Gene, 268(1-2): 67-75.
[11] Crisafi F, Denaro R, Genovese M, et al.2011.Comparison of 16S rDNA and toxR genes as targets for detection of Vibrio anguillarum in Dicentrarchus labrax kidney and liver[J].Research in Microbiology, 162(3): 223-230.
[12] Eppler E, Berishvili G, Mazel P, et al.2010.Distinct organ-specific up- and down-regulation of IGF-Ⅰ and IGF-ⅡmRNA in various organs of a GH-overexpressing transgenic Nile tilapia[J] Transgenic Research, 19(2): 231-240.
[13] Geertz R, Kiess W, Kessler U, et al.1997.Expression of IGF receptors on alveolar macrophages: IGF-induced changes in InsPi formation, [Ca2+]i, and pHi[J].Molecular & Cellular Biochemistry, 177(1-2): 33-45.
[14] González-Stegmaier R, Guzmán F, Albericio F, et al.2015.A synthetic peptide derived from the D1 domain of flagellin induced the expression of proinflammatory cytokines in fish macrophages[J].Fish & Shellfish Immunology, 47(1): 239-244.
[15] Guan F, Lu X J, Li C H, et al.2017.Molecular characterization of mudskipper (Boleophthalmus pectinirostris) hypoxia-inducible factor-1α (HIF-1α) and analysis of its function in monocytes/macrophages[J].PLoS One, 12(5): e0177960.
[16] He Y Q, Chen J, Lu X J, et al.2013.Characterization of P2X7R and its function in the macrophages of ayu, Plecoglossus altivelis[J].PLoS One, 8(2): e57505.
[17] Hua Q X, Mayer J P, Jia W, et al.2006.The folding nucleus of the insulin superfamily: A flexible peptide model foreshadows the native state[J].Journal of Biological Chemistry, 281(38): 28131-28142.
[18] Irmawati, Alimuddin, Zairin M, et al.2016.Molecular cloning and sequence analysis of insulin-like growth factors-1 cDNA of giant gourami, Osphronemus goramy[J].Aquaculture, Aquarium, Conservation & Legislation-International Journal of the Bioflux Society, 9(1): 174-183.
[19] Jansen M, van Schaik F M, Ricker A T, et al.1983.Sequence of cDNA encoding human insulin-like growth factor Ⅰ precursor[J].Nature, 306(5943): 609-611.
[20] Jiang Y, Husain M, Qi Z, et al.2015.Identification and expression analysis of two interleukin-23α (p19) isoforms, in rainbow trout Oncorhynchus mykiss and Atlantic salmon Salmo salar[J].Molecular Immunology, 66(2): 216-228.
[21] Jie C, Qiang C, Lu X J, et al.2016.The protection effect of LEAP-2 on the mudskipper (Boleophthalmus pectinirostris) against Edwardsiella tarda infection is associated with its immunomodulatory activity on monocytes/macrophages[J].Fish & Shellfish Immunology, 59: 66-76.
[22] Kai J E, Clifford M A, Hedrick R P, et al.2008.Expression of immune-regulatory genes in juvenile Chinook salmon following exposure to pesticides and Infectious hematopoietic necrosis virus (IHNV)[J].Fish & Shellfish Immunology, 25(5): 508-516.
[23] Kashima H, Shiozawa T, Miyamoto T, et al.2009.Autocrine stimulation of IGF1 in estrogen-induced growth of endometrial carcinoma cells: Involvement of the mitogen-activated protein kinase pathway followed by up-regulation of cyclin D1 and cyclin E[J].Endocrine Related Cancer, 16(1): 113-122.
[24] Lawrence T, Natoli G.2011.Transcriptional regulation of macrophage polarization: Enabling diversity with identity[J].Nature Reviews Immunology, 11(11): 750-761.
[25] Lu X J, Chen Q, Rong Y J, et al.2017.CXCR3.1 and CXCR3.2 differentially contribute to macrophage polarization in teleost fish[J].Journal of Immunology, 198(12): 4692-4706.
[26] LU X J, Zhang H, Yang G J, et al.2016.Comparative transcriptome analysis on the alteration of gene expression in ayu (Plecoglossusal tivelis) larvae associated with salinity change[J].Zoological Research, 37(3): 126-135.
[27] Ma Q, Liu S F, Zhuang Z M, et al.2011.Molecular cloning, expression analysis of insulin-like growth factor Ⅰ (IGF-Ⅰ) gene and IGF-Ⅰ serum concentration in female and male Tongue sole (Cynoglossus semilaevis)[J].Comparative Biochemistry & Physiology Part B, 160(4): 208-214.
[28] Ma W, Wu J, Zhang J, et al.2016.Sex differences in the expression of GH/IGF axis genes underlie sexual size dimorphism in the yellow catfish (Pelteobagrus fulvidraco)[J].Science China Life Sciences, 59(4): 431-433.
[29] O'Connor J C, Mccusker R H, Strle K, et al.2008.Regulation of IGF-I function by proinflammatory cytokines: At the interface of immunology and endocrinology[J].Cellular Immunology, 252(1): 91-110.
[30] Pérez L, Ortiz-Delgado J B, Manchado M.2016.Molecular characterization and transcriptional regulation by GH and GnRH of insulin-like growth factorsⅠandⅡin white seabream (Diplodus sargus)[J].Gene, 578(2): 251-262.
[31] Piazzon M C, Savelkoul H F J, Pietretti D, et al.2015.Carp ⅠIL10 has anti-inflammatory activities on phagocytes, promotes proliferation of memory T cells, and regulates B cell differentiation and antibody secretion[J].The Journal of Immunology, 194(1): 187-199.
[32] Ponce M, Infante C, Funes V, et al.2008.Molecular characterization and gene expression analysis of insulin-like growth factors Ⅰ and Ⅱ in the redbanded seabream, Pagrus auriga: Transcriptional regulation by growth hormone[J].Comparative Biochemistry & Physiology Part B Biochemistry & Molecular Biology, 150(4): 418-426.
[33] Sica A, Mantovani A.2012.Macrophage plasticity and polarization: In vivo veritas[J].Journal of Clinical Investigation, 122(3): 787-795.
[34] Sitjàbobadilla A, Calduchginer J, Saeravila A, et al.2008.Chronic exposure to the parasite Enteromyxum leei (Myxozoa: Myxosporea) modulates the immune response and the expression of growth, redox and immune relevant genes in gilthead sea bream, Sparus aurata L.[J].Fish & Shellfish Immunology, 24(5): 610-619.
[35] Spadaro O, Camell C D, Bosurgi L, et al.2017.IGF1 shapes macrophage activation in response toimmunometabolic challenge[J].Cell Reports, 19(2): 225-234.
[36] Tamura K, Peterson D, Peterson N, et al.2011.MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods[J].Molecular Biology & Evolution, 28(10): 2731-2739.
[37] Tsai T C, Shih C C, Chien H P, et al.2018.Anti-apoptotic effects of IGF-Ⅰ on mortality and dysmorphogenesis in tbx5-deficient zebrafish embryos[J].BMC Developmental Biology, 18(1): 5-20.
[38] Vikeså V, Nankervis L, Remø S C, et al.2015.Pre and postprandial regulation of ghrelin, amino acids and IGF1 in Atlantic salmon (Salmo salar L.) at optimal and elevated seawater temperatures[J].Aquaculture, 438(438): 159-169.
[39] Vong Q P, Chan K M, Cheng C H.2003.Quantification of common carp (Cyprinus carpio) IGF-Ⅰ and IGF-Ⅱ mRNA by real-time PCR: Differential regulation of expression by GH[J].Journal of Endocrinology, 178(3): 513-521.
[40] Wallenius K, Sjögren K, Peng X D, et al.2001.Liver-derived IGF-Ⅰ regulates GH secretion at the pituitary level in mice[J].Endocrinology, 142(11): 4762-4770.
[41] Wenger M, Shved N, Akgül G, et al.2014.Developmental oestrogen exposure differentially modulates IGF-Ⅰ and TNF-α expression levels in immune organs of Yersinia ruckeri-challenged young adult rainbow trout (Oncorhynchus mykiss)[J].General & Comparative Endocrinology, 205: 168-175.
[42] Wu J, Shi Y H, Zhang X H, et al.2015.Molecular characterization of an IL-1β gene from the large yellow croaker (Larimichthys crocea) and its effect on fish defense against Vibrio alginolyticus infection[J].Zoological Research, 36(3): 133-141.
[43] Xu X G, Hu J F, Ma J X, et al.2016.Essential Roles of TIM-1 and TIM-4 homologs in adaptive humoral immunity in a zebrafish model[J].Journal of Immunology, 196(4): 1686-1700.
[44] Yildirimaksoy M, Beck B H.2017.Antimicrobial activity of chitosan and a chitosan oligomer against bacterial pathogens of warmwater fish[J].Journal of Applied Microbiology, 122(6): 1570-1578. |
|
|
|
