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Distribution and Expression of FSHR and LHR in the Uterus of Ganjia Tibetan Sheep (Ovis aries) During Estrus Cycle |
WANG Xia, GE Wen-Bo, HE Yu-Qin*, YANG Da-Peng, ZHOU Kai-Ren, CHEN Wei-Gang, BAO Ying-Ying |
College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China |
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Abstract Ganjia Tibetan sheep (Ovis aries) grow at high altitude and has a low reproductive rate. The aim of this study was to elucidate the gene expression and tissue distribution of follicle stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) in the uterus of the estrous cycle of Ganga Tibetan sheep, to explore the regulation of FSH and LH in Tibetan sheep breeding activities. In the experiment, 32 healthy and non-pregnant Ganjia Tibetan sheep were selected and the uteruses were collected at different stages of the estrous cycle. The detection results of qRT-PCR showed that FSHR and LHR were expressed in uterus tissue during the estrous cycle of Ganjia Tibetan sheep, and the expression level of FSHR was the highest in Estrus, which was significantly higher than that in Proestrus and Metaestrus (P<0.01), and the expression level of LHR was the highest in Diestrus, which was significantly higher than that in Proestrus and Estrus (P<0.01). The detection results of immunohistochemistry showed that FSHR and LHR distributed in the Cervix, Corpus uteri and Cornual uteri of Ganjia Tibetan sheep during estrus cycle, mainly distributed in stromal cells, glandular epithelial cells, vascular endothelial cells and myometrial smooth muscle cells, and FSHR had the most distribution in glandular epithelial cells, stromal cells and myometrial smooth muscle cells in Estrus, and the most distribution in vascular endothelial cells in Diestrus; LHR had the most distribution in glandular epithelial cells, stromal cells and myometrial smooth muscle cells in Diestrus, and the most distribution in vascular endothelial cells in the Metaestrus. Above results indicated that FSHR and LHR might be involved in the regulation of the physiological function of Tibetan uterus during the estrus cycle. This study provides reference data for studying the regulation mechanisms of reproductive activity in Tibetan sheep at the cellular and molecular levels.
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Received: 15 February 2019
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Corresponding Authors:
heyq@gsau.edu.cn
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1 阿依木古丽. 2011. 发情周期不同阶段牦牛子宫中生殖激素受体表达的研究[D]. 博士学位论文, 甘肃农业大学, 导师: 崔燕, pp. 61-78. (Ayimuguli.2011. Different expression of reproductive hormone receptors in yak uterus during estrous cycle[D]. Thesis for Ph.D., Gansu Agriculturial University, Supervisor: Cui Y, pp. 61-78.) 2 成令忠, 钟翠萍, 蔡文琴. 2003. 现代组织学[M]. 上海: 上海科学技术文献出版社, 2003: 1022-1032. (Cheng L Z, Zhong C P, Cai W Q.2003. Modern Histology[M]. Shanghai: Shanghai Science and Technology Literature Publishing House, 2003: 1022-1032.) 3 付瑶, 姜昊, 袁宝, 等. 2017. BMP15促进牛颗粒细胞FSH受体表达并调控其激素分泌[J]. 吉林畜牧兽医, 38(12): 6-8. (Fu Y, Jiang H, Yuan B, et al.2017. BMP15 promotes the expression of FSH receptor in bovine granulosa cells and regulates its hormone secretion[J]. Jilin animal husbandry and veterinary, 38(12): 6-8.) 4 韩志磊, 史军红, 何玉琴, 等. 2016. 甘加型藏羊发情周期阴道细胞变化规律[J]. 中国兽医学报, 36(7): 1241-1246. (Han Z L, Shi J H, He Y Q, et al.2016. Observation on the changes of vaginal cells of Ganjia Tibetan sheep during the estrous cycle[J]. Chinese Journal of Veterinary Science, 36(7): 1241-1246.) 5 胡威, 崔燕, 潘阳阳, 等. 2015. 黄体生成素受体基因在牦牛发情周期不同阶段生殖系统中的表达模式[J]. 生物技术通报, 31(9): 232-237. (Hu W, Cui Y, Pan Y Y, et al.2015. Analysis of expression pattern of LHR Gene in the reproductive system of Yak during the estrous cycle[J]. Biotechnology Bulletin, 31(9): 232-237.) 6 梁鸿雁, 周虚, 李纯绵, 等. 2009. 促卵泡激素受体和黄体素受体在猪子宫中的定位研究[J]. 中国畜牧兽医, 36(4): 125-128. (Liang H Y, Zhou X, Li C M, et al.2009. Immunohistochemical localization FSHR and LHR on porcine uterus[J]. China Animal Husbandary and Veterinary Medicine, 36(4): 125-128.) 7 刘丹阳. 2014. 母犬发情周期中生殖器官内GTHR的分布及其mRNA半定量表达的研究[D]. 硕士学位论文, 延边大学, 导师: 崔成都, pp. 19-22. (Liu D Y.2014. The localization of GTHR and semiquantitative expression of GTHR mRNA during estrous cycle in the reproductive tissues in bitches[D]. Thesis for M.S., Yanbian University, Supervisor: Cui C D, pp. 19-22.) 8 申颖, 王慧, 王树迎, 等. 2009. LHR基因在济宁青山羊发情周期不同阶段子宫中表达差异的研究[J]. 畜牧兽医学报, 40(3): 338-342. (Shen Y, Wang H, Wang S Y, et al.2009. Different expression of LHR mRNA in the uterus of four estrous cycle phases in Jining gray goat[J].Acta Veterinaria et Zootechnica Sinica,40(3): 338-342. ) 9 史军红, 何玉琴, 华道才让, 等. 2017. 甘加藏羊发情周期腺垂体和血浆中GTH含量动态变化[J]. 中国兽医学报, 37(10): 1998-2003. (Shi J H, He Y Q, Hua C D R, et al.2017. The dynamic changes of GTH content in pituitary and plasma in estrous cycle of Ganjia Tibetan Sheep[J]. Chinese Journal of Veterinary Science, 37(10): 1998-2003. ) 10 石中强, 徐艳, 王树迎, 等. 2012. 沂蒙黑山羊子宫中FSHR和LHR基因在发情周期内的表达[J].山东农业大学学报, 43(1): 48-54. (Shi Z Q, Xu Y, Wang S Y, et al.2012. mRNA experssion regularity of FSHR gene and LHR gene in uterus of the estrous cycle in Yimeng black goat[J]. Journal of Shandong Agricultural University (Natural Science), 43(1): 48-54.) 11 魏军强. 2009. 高繁山羊发情周期不同阶段子宫FSHR mRNA表达的定量分析及FSHR免疫阳性细胞分析研究[D]. 硕士学位论文, 山东农业大学. pp. 35. (Wei J Q. 2009. Quantitative analysis of FSHR mRNA and FSHR immunoreactive cells distribution in the uterus of high fecundity goat of different estrous cycle[D]. Thesis for M.S., Shangdong Agricultural University. pp. 35.) 12 向泽宇, 王长庭. 2011. 青藏高原藏羊遗传资源的现状、存在问题及对策[J]. 中国畜牧兽医文摘, 27(2): 1-4. (Xiang Z Y, Wang C T.2011. The present situation exciting problems and countermeasures of genetic resources of Tibetan sheep in qinghai-tibet plateau[J]. Chinese animal husbandry and veterinary abstract, 27(2): 1-4.) 13 张贺. 2007. 不同脉冲频率GnRH刺激对大鼠垂体细胞中FSHβ mRNA和LHβ mRNA表达水平的影响[D]. 硕士学位论文, 吉林大学, 导师: 周虚, pp. 46-71. (Zhang H.2007. Diffierent effects of gonadotropin-releasing hormone (GnRH) pulse frequency on gonadotropin β subunit mRNA levels in vitro[D]. Thesis for M.S., Jilin Uuiversity, Supervisor: Zhou X, pp. 46-71.) 14 朱雪雁, 葛闻博, 何玉琴, 等. 2017. 甘加藏羊发情周期不同阶段血浆生殖激素分泌规律[J]. 河南农业大学, 46(11): 138-143. (Zhu X Y, Ge W B, He Y Q, et al.2017. Dynamics research of reproductive hormone secretion in the plasma of Ganjia Tibetan sheep in different stages of estrous cycle[J]. Journal of Henan Agricultural Sciences, 46(11): 138-143.) 15 Anna T, Grazul-Bilska, Arshi Reyaz, et al.2018. Follicle stimulating hormone receptor protein is expressed in ovine uterus during the estrous cycle and utero-placenta during early pregnancy: An immunohistochemical study[J]. Acta Histochemica, 120(5): 420-428. 16 Lin P C, Li X, Lei Z M, et al.2003. Human cervix contains functional luteinizing hormone/human chorionic gonadotropin receptors[J]. Journal of Clinical Endocrinology & Metabolism, 88(7): 3409-3414. 17 Méndez Eva, Montserrat Núria, Planas Josep V.2004. Modulation of the steroidogenic activity of luteinizing hormone by insulin and insulin-like growth factor-I through interaction with the cAMP-dependent protein kinase signaling pathway in the trout ovary[J]. Molecular & Cellular Endocrinology, 229(1-2): 49-56. 18 Abel M H, Widen A, Wang X, et al.2014. Pituitary gonadotrophic hormone synthesis, secretion, subunit gene expression and cell structure in normal and follicle-stimulating hormone β knockout, follicle-stimulating hormone receptor knockout, luteinising hormone receptor knockout, hypogonadal and ovariectomised female mice[J]. Journal of Neuroendocrinology, 26(11): 785-795. 19 Saleh M, Shahin M, Wuttke W, et al.2012. Pharmacokinetics of human chorionic gonadotropin after i. m. administration in goats (Capra hircus)[J]. Reproduction, 144(1): 77-81. 20 Ku S Y, Choi Y M, Suh C S, et a1.2002. Effect of gonadotropins on human endometrial stromal cell proliferation in vitro[J]. Archives of Gynecology and Obstetrics, 266(4): 223-228. 21 Leethongdee S, Khalid M, Bhatti A, et a1.2007. The effects of the prostaglandin E analogue Misoprostol and follicle-stimulating hormone on cervical penetrability in ewes during the peri-ovulatory period[J]. Theriogenology, 67(4): 767-777. 22 Toth P.2001. Clinical data supporting the importance of vascular LH/hCG receptors of uterine blood vessels[J]. Seminars in Reproductive Medicine, 19(1): 55-61. 23 Ziecik A J, Bodek G, Biltek A.2005. Nongonadal LH receptors,their involvement in femail reproductive function and a new applicable approach[J]. Vet, 169(1): 75-84. 24 Ziecik A J, Stepien A, Gawronska B.2000. Importance of endometrial luteinizing hormone receptors in induction of luteolysis and maternal recognition of pregnancy in the pig[J]. Reproduction in Domestic Animals, 35(3): 190-192. |
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