Association Analysis Between Polymorphisms of Follicle Stimulating Hormone Receptor Gene and Egg Production in White King Pigeon (Columba livia)
MAO Yue-Jiao1,2, LI Guo-Qin2,3, CHEN Xiao-Yan4, LI Gao-Lou5, WEN Ji-Hui4, TAO Zheng-Rong2, LI Hai-Ying1,*, LU Li-Zhi2,3,*
1 College of Animal Science, Xingjiang Agricultural University, Urumqi 830052, China;
2 Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310000, China;
3 Key Laboratory of Information Traceability for Agricultural Products, Ministry of Agriculture of China, Hangzhou 301121, China;
4 Pingyang County Aofeng Pigeon Industry Co., Ltd., Wenzhou 325400, China;
5 Pingyang County Pigeon Association, Wenzhou 325400, China
Abstract The follicle stimulating hormone receptor (FSHR) is used to mediate the physiological functions of biomacromolecule follicle stimulating hormone, and has an important influence on the reproductive traits of animals. In order to analyze the relationship between single nucleotide polymorphisms (SNPs) of FSHR gene and egg production in White King Pigeon (Columba livia), this study selected 209 pigeons as the research subjects and used the direct sequencing of PCR products to screen the SNPs of exon 6, exon 9, exon 10 and their adjacent upstream and downstream sequence in FSHR gene. The results showed that 18 mutation sites were found in the 3 fragments detected and the amino acid sequences were changed accordingly. Among them, 3 mutation sites G67729A, A67801G, A67898G were found in exon 6 and its upstream and downstream sequences. 5 mutation sites C76462T, T76468C, G76527T, C76616T, T76651G were found in exon 9 and its upstream and downstream sequences. 10 mutation sites were found in exon 10, which were A82714G, C82930T, A83083C, G83098T, G83158A, C83200T, A83246C, T83395C, T83740C, C83766T. T76468C and C83766T had 2 genotypes AA, AB, and the rest had 3 genotypes: AA, AB, BB. A Hardy-Weinberg equilibrium test was performed on 18 loci and found to be in equilibrium except for the C76616T locus. Correlation analysis between polymorphic loci and average egg production showed that the homozygous BB genotype of A67801G locus was extremely significantly correlated with egg production (P<0.01), and the homozygous BB genotype of C76616T locus was significantly correlated with egg production (P<0.05). Therefore, the A67801G and C76616T loci in the FSHR gene could be utilized as candidate molecular marker sites for the selection and breeding of the egg-laying traits of White King Pigeons, which would provide a theoretical basis for marker-assisted breeding from the molecular level to better improve the breeding performance of the pigeon population and shorten the breeding years.
MAO Yue-Jiao,LI Guo-Qin,CHEN Xiao-Yan, et al. Association Analysis Between Polymorphisms of Follicle Stimulating Hormone Receptor Gene and Egg Production in White King Pigeon (Columba livia)[J]. 农业生物技术学报, 2019, 27(8): 1452-1459.
[1] 陈祥, 龙威海, 孙振梅, 等. 2017. 贵州地方山羊FSHR基因与繁殖性状的相关性研究[J]. 农业生物技术学报, 25(01): 94-101.
(Chen X, Long W H, Sun Z M, et al.2017. Correlation between FSHR gene and reproductive traits in Guizhou local goats[J]. Journal of Agricultural Biotechnology, 25(01): 94-101.)
[2] 李亨, 陈宽维, 李慧芳, 等. 2009. 文昌鸡促卵泡素受体(FSHR)基因外显子区域SNPs分析[J]. 中国畜牧兽医, 36(07): 83-87.
(Li H, Chen K W, Li H F, et al.2009. Single nucleotide polymorphisms on follicle stimulating hormone receptor (FSHR) gene of Wenchang chicken[J]. China Animal Husbandry & Veterinary Medicine, 36(07): 83-87.)
[3] 陶争荣, 徐小钦, 董丽艳, 等. 2016. 蛋鸽雌激素受体基因多态性与产蛋量关联分析[J]. 农业生物技术学报, 24(3): 373-379.
(Tao Z R, Xu X X, Dong L Y, et al.2016. The single nucleotide polymorphism of ESR genes associated with egg production in laying pigeon[J]. Journal of Agricultural Biotechnology, 24(3): 373-379.)
[4] 辛清武, 缪中纬, 李丽, 等. 2016. 金定鸭FSHR基因第1~7外显子的克隆及SNP位点的筛查[J]. 福建农业学报, 31(12): 1262-1266.
(Xin Q W, Miu Z W, Li L, et al.2016. Cloning and polymorphisms of 1~(st)-7~(th) exons in FSHR of Jinding Ducks[J]. Fujian Journal of Agricultural Sciences, 31(12): 1262-1266. )
[5] 徐小钦, 陈黎, 李进军, 等. 2015. 微卫星标记技术鉴定某保种场四川白鹅保种效果[J]. 浙江农业学报, 27(05): 751-755.
(Xu X X, Chen L, Li J J, et al.2015. Identification of Sichuan white goose seeding effect in a protected field by microsatellite marking technique[J]. Acta Agriculturae Zhejiangensis, 27(05): 751-755.)
[6] 肖同乾, 鲁翠云, 李超, 等. 2013. 8种鲤养殖品种线粒体Cyt b基因的遗传多样性和系统进化分析[J]. 水产学报, 37(03): 344-350.
(Xiao T Q, Lu C Y, Li C, et al.2013. Genetic diversity and phylogenetic analysis of mitochondrial Cyt b gene in eight species of alfalfa cultivars[J]. Journal of Fisheries of China, 37(03): 344-350.)
[7] 张丽萍, 刘雅丽, 熊化鑫, 等. 2016. 灵昆鸡VLDLR和CTSD基因多态性与产蛋性状的相关性研究[J]. 中国农学通报, 32(26): 6-14.
(Zhang L P, Liu Y L, Xiong H X, et al.2016. Correlation between VLDLR and CTSD gene polymorphisms and egg production traits in Lingkun chicken[J]. Chinese Agricultural Science Bulletin, 32(26): 6-14.)
[8] Chu M X, Guo X H, Feng C J, et al.2012. Polymorphism of 5′ regulatory region of ovine FSHR gene and its association with litter size in Small Tail Han sheep[J]. Molecular Biology Reports, 39(4): 3721-3725.
[9] Houde A, Lambert A, Saumande J, et al.1994. Structure of the bovine follicle-stimulating hormone receptor complementary DNA and expression in bovine tissues[J]. Molecular Reproduction & Development, 39: 127-135
[10] Kang B, Jiang D M, Zhou R J, et al.2010. Expression of follicle-stimulating hormone receptor (FSHR) mRNA in the ovary of Zi Geese during developmental and egg laying stages[J]. Folia Biologica, 58(1-2): 61-66.
[11] Minegishi T, Nakamura K, Takakura Y, et al.1991. Cloning and sequencing of human FSH receptor cDNA[J]. Biochemical & Biophysical Research Communications, 175(3): 1049-54.
[12] Lussiana C, Guani B, Mari C, et al.2008. Mutations and polymorphisms of the FSH receptor (FSHR) gene: Clinical implications in female fecundity and molecular biology of FSHR protein and gene[J]. Obstetrical & Gynecological Survey, 63(12): 785-95.
[13] O'Shaughnessy P J, Dudley K, Rajapaksha W R.1996. Expression of follicle stimulating hormone-receptor mRNA during gonadal development[J]. Molecular & Cellular Endocrinology, 125(1-2): 169-175.
[14] Sprengel R, Braun T, Nikolics K, et al.1990. The testicular receptor for follicle stimulating hormone: Structure and functional expression of cloned cDNA[J]. Molecular Endocrinology, 4(4): 525-530.
[15] Tisdall D J, Watanabe K, Hudson N L, et al.1995. FSH receptor gene expression during ovarian follicle development in sheep[J]. Journal of Molecular Endocrinology, 15(3):273-81.
[16] Wu J S, Zhang G X, Dai G J, et al.2012. Polymorphism of exon1 of FSHR gene and its relationship with litter size in Xiaomeishan Pigs[J]. Chinese Journal of Animal & Veterinary Sciences, 43(4): 509-515.
[17] Wang K H, Guo J, Dou T C, et al.2012. Association analyses of single nucleotide polymorphisms in intron 2 of FSHR gene with reproductive traits in Rugao Yellow Chicken[J]. China Animal Husbandry & Veterinary Medicine, 39(5): 152-156.
[18] Xu J G, Gao X F, Li X, et al.2017. Molecular characterization, expression profile of the FSHR, gene and its association with egg production traits in Muscovy Duck[J]. Journal of Genetics, 96(2): 341-351.
[19] Yang A L, Yang K, Wu M L.2014. The relationship between polymorphism of FSHβ, POU1F1,FSHR gene and egg production traits in Tibetan Chickens[J]. Journal of Southwest University for Nationalities, 40(01): 1-10.
[20] You S, Bridgham J T, Foster D N, et al.1996. Characterization of the chicken follicle-stimulating hormone receptor (cFSH-R) complementary deoxyribonucleic acid, and expression of cFSH-R messenger ribonucleic acid in the ovary[J]. Biology of Reproduction, 55(5): 1055-1062.
[21] Zhang X D, Zhu H Y, Zhou J, et al.2015. Relationship between polymorphisms in exon 10 of FSHR gene and litter size in swine[J]. Genetics & Molecular Research, 14(3): 8252-8261.
