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| Study on PRLR Gene Expression and Tissue Distribution in Pituitary and Ovary of Ganjia Tibetan Sheep (Ovis aries) During Estrous Cycle |
| ZHOU Kai-Ren1,2, HE Yu-Qin1,*, GE Wen-Bo1, ZHANG Quan-Wei1, YANG Da-Peng1, WANG Xia1, CHEN Wei-Gang1, BAO Ying-Ying1 |
1 College of Life Sciences and Technolog, Gansu Agricultural University, Lanzhou 730070, China;
2. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China |
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Abstract Ganjia Tibetan sheep (Ovis aries) are endemic animal in Gansu, and the reproduction rate is low due to the particularity of their living environment. In order to study the expression and distribution of prolactin receptor (PRLR) in pituitary and ovarian tissues of Ganjia Tibetan sheep during estrous cycle and illuminate the regulation of gene expression in reproductive activity, 32 Ganjia Tibetan sheep were selected, and the expression and distribution of PRLR in pituitary and ovarian tissues during estrous cycle were detected by immunohistochemical staining and real-time fluorescent quantitative PCR. The results showed that PRLR and its gene expression were found in the pituitary and ovarian tissues of estrous cycle of Ganjia Tibetan sheep. In the pituitary, the gene expression of PRLR in oestrus was the highest, which was significantly higher than that in the metestrus (P<0.05); PRLR-positive cells were mainly distributed in the cytoplasm of the distal eosinophils of the pituitary gland, and PRLR had the most distribution in the proestrus and the least in the oestrus. In the ovarian, PRLR gene expression was highest in oestrus, significantly higher than that in metestrus, proestrus and diestrus (P<0.05); Positive PRLR cells were mainly distributed in follicular granule layer, and had the most distribution in proestrus and the least in estrus period, and the pre-estrus and inter-estrus period had more distribution than that in post-estrus period and estrus period (P<0.05).The distribution and gene expression of PRLR in pituitary and ovary tissues of Ganga Tibetan sheep during estrous cycle were different, suggesting that there might be differences in the regulation of PRLR on estrous cycle. The results of this study provide basic data for the regulation of reproductive performance of Tibetan sheep.
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Received: 04 March 2019
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Corresponding Authors:
heyq@gsau.edu.cn
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1 曹果清, 李步高, 刘建华, 等. 2006. 猪催乳素受体基因对繁殖性能的影响[J]. 中国畜牧杂志, 42(13): 45-46, 60.
(Cao G Q, Li B G, Liu J H, et al. 2006. Effect of prolactin receptor gene on reproductive performance in swine[J], Chinese Journal of Animal Science, 42(13): 45-46, 60.)
2 葛闻博. 2018. 高原甘加藏羊发情周期下丘脑GnRH的动态分泌及其受体在垂体和卵巢中表达[D]. 硕士学位论文, 甘肃农业大学, 导师: 何玉琴, pp. 47-58.
(Ge W B.2018. Secretion of hypothalamus GnRH and the expression of GnRHR in the pituitary and ovary during the estrus cycle of Ganjia Tibetan sheep[D]. Thesis for M.S., Gansu Agricultural University, Supervisor: He Y Q, pp. 47-58.)
3 韩志磊, 史军红, 何玉琴, 等. 2016. 甘加型藏羊发情周期阴道细胞变化规律[J]. 中国兽医学报, 36(7): 1241-1246.
(Han Z L, Shi J H, He Y Q, et al.2016. Observation on the changes of vaginal cells of Ganjia Tibetan sheep during the estrous cycle[J]. Chinese Veterinary Science, 36(7): 1241-1246.)
4 胡威, 陈虹, 潘阳阳, 等. 2015. LHR在牦牛肾和卵巢中表达情况的研究[J]. 中国兽医科学, 45(02): 212-216.
(Hu W, Chen H, Pan Y Y.et al.2015. Study on expression of luternizing hormone receptor in the kidney and ovary of yak[J]. Chinese Veterinary Science, 45(02): 212-216.)
5 郎多勇, 李发弟, 郎侠, 等. 2010. 甘加型藏羊种质特性研究[J]. 畜牧兽医杂志, 29(03): 31-34.
(Lang D Y, Li F D, Lang X, et al.2010. Study on species property of Ganjia Tibetan sheep[J]. Acta Veterinaria et Zootechnica Sinica, 29(03): 31-34.)
6 李晓雨, 贺小云, 刘秋月, 等. 2018. TAC1和PRLR基因在绵羊不同繁殖状态下的表达模式分析[J]. 畜牧兽医学报, 49(02): 253-262.
(Li X Y, He X Y, Liu Q Y, et al.2018. Expression pattern analysis of TAC1 and PRLR in different reproductive states of sheep[J]. Acta Veterinaria et Zootechnica Sinica, 49(02): 253-262.)
7 生冉, 闫素梅. 2014. 催乳素与其他激素对乳腺内乳成分合成的协同调节作用[J]. 动物营养学报, 26(6): 1435-1443.
(Sheng R, Yan S M.2014. Prolactin and other hormones synergistic action on regulating milk composition synthesis in mammary glands[J]. Chinese Journal of Animal Nutrition, 26(6): 1435-1443.)
8 谭敦勇, 彭湘萍. 2012. 催乳素受体研究[J]. 生理科学进展, 43(01): 17-23.
(Tan D Y, Peng X P.2012. Progress in Prolactin receptor research[J]. Progress in Physiological Sciences, 43(01): 17-23)
9 王文芳. 2016. 高原甘加型藏羊发情周期卵巢FSHR和LHR分布及其mRNA表达规律研究[D]. 硕士学位论文, 甘肃农业大学, 导师: 何玉琴, pp. 46-55.
(Wang W F.2016. The distribution and mRNA expression of FSHR and LHR in the ovary of Ganjia Tibetan sheep in the estrous cycle[D]. Thesis for M.S., Gansu Agricultural University, Supervisor: He Y Q, pp. 46-55.)
10 熊东升, 吕颜枝, 代盈盈, 等. 2012. 妊娠山羊腺垂体中ER与PRL、FSH和LH的共存性研究[J]. 动物医学进展, 33(10): 33-36.
(Xiong D S, Lu Y Z, Dan Y Y, et al.2012. Study on the coexistence of estrong receptor with PRL, FSH and LH in adenohypophysis of pregnancy goats[J]. Progress in Veterinary Medicine, 33(10): 33-36.)
11 余四九, 张家骅. 2013. 兽医产科学[M]. 精简版. 北京: 中国农业出版社, pp. 5-23.
(Yu S J, Zhang J Y.2013. Veterinary obstetrics[M]. Lite version. Beijing: China agriculture press, pp. 5-23.)
12 赵敏, 连小龙, 刘景楠, 等. 2018. 不同施加因素对下丘脑-垂体-卵巢轴调节作用的实验研究概况[J]. 甘肃中医药大学学报, 35(03): 92-96.
(Zhan M, Lian X L, Liu J N, et al.2018. Overview of experimental study on regulation effect of different exterior factors on hypotha-lamic-pituitary-ovarian axis[J]. Gansu University Of Chinese Medicine, 35(03): 92-96.)
13 赵强. 2015. 绵羊PRLR、PRLH和PRLHR基因的克隆及其在下丘脑-垂体-性腺中表达的研究[D]. 硕士学位论文, 甘肃农业大学, 导师: 马友记, pp: 27-30.
(Zhao Q.2015. Molecular cloning of PRLR, PRLH and PRLHR gene in ovine and theirs expression in the hypothalamus, pituitary, uterus and ovary[D]. Thesis for M.S., Gansu Agricultural University, Supervisor: Ma Y J, pp: 27-30.)
14 赵秀华, 储明星, 刘荣志, 等. 2007. 催乳素基因的研究进展[J]. 中国畜牧兽医, 34(10): 28-33.
(Zhao X H, Chu M X, Liu R Z, et al.Progress on prolactin gene[J]. China animal husbandry and veterinary, 34(10): 28-33.)
15 郑宇新, 张妮娜, 崔亚娟, 等. 2018. 雌激素受体在卵泡发育中作用的研究进展[J]. 黑龙江畜牧兽医, (01): 60-62.
(Zheng Y X, Zhang N N, Cui Y J, et al. 2018. Progress of estrogen receptors effect on follicular development[J]. Heilongjiang Animal Science and Veterinary Medicine, (01): 60-62.)
16 Bole-Feysot C, Goffin V, Edery M, et al.1998. Prolactin (PRL) and its receptor: actions, signal transduction pathways and phenotypes observed in PRL receptor knockout mice[J]. Endocrine Reviews, 19(3): 225-268.
17 Bratthauer G L, Stamatakos M D, Vinh T N.2010. Cells with minimal expression of the JAK/STAT pathway related proteins STAT5a and the prolactin receptor: Evidence of an alternate prolactin receptor isoform in breast disease[J]. Protein and Peptide Letters, 17(1): 104-108.
18 Clevenger C V, Gadd S L, Zheng J.2009. New mechanisms for PRLr action in breast cancer[J]. Trends in Endocrinology & Metabolism, 20(5): 223-229.
19 Clarke D L, Arey B J, Linzer D I.1993. Prolactin receptor messenger ribonucleic acid expression in the ovary during the rat estrous cycle[J]. Endocrinology, 133(6): 2594-2603.
20 García-Martínez J M, Calcabrini A, González L, et al.2010. Anon-catalytic function of the Src family tyrosine kinases controls prolactin-induced Jak2 signaling[J]. Cell Signal, 22(3): 415-426.
21 Nakamura E, Otsuka F, Inagaki K, et al.2010. A novel antagonistic effect of the bone morphogenetic protein system on prolactin actions in regulating steroidogenesis by granulosa cells[J]. Endocrinology, 151(11): 5506-5518.
22 Simon-Holtorf J, Mönig H, Klomp H J, et al.2006. Expression and distribution of prolactin receptor in normal, fibrotic, and cirrhotic human liver[J]. Experimental and Clinical Endocrinology & Diabetes, 114(10): 584-589.
23 Shao R, Nutu M, Weigdegard B, et al.2008. Differences inprolactin receptor (PRLR) in mouse and human fallopian tubes: Evidence for multiple regulatory mechanisms controlling PRLR isoform expression in mice[J]. Biology of Reproduction, 79(4): 748-757. |
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