HIF1α和Beclin1在成年牦牛脑组织中的表达与分布

doi:10.3969/j.issn.1674-7968.2019.10.018

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Abstract The central nervous system is an important regulatory system for the movement of various organs of the body. And the brain as a major part plays an important role in the animal growth and adaptation to the hypoxic environment. In order to investigate the expression of hypoxia-inducible factor 1α (HIF1α) and homolog of yeast ATG6 Beclin1 and their relationship between the process of hypoxia adaptation of adult yak (Bos grunniens) brain tissues, the expression of HIF1α and Beclin1 mRNA and protein in adult yak brain tissues were detected by qRT-PCR, ELISA and immunohistochemistry (IHC). The results of qRT-PCR and ELISA showed that the mRNA and protein levels of HIF1α and Beclin1 were the highest in adult yak hippocampus, followed by cerebral cortex, corpora quadrigemina, medulla oblongata and cerebellum. Immunohistochemistry staining showed that HIF1α and Beclin1 positive products were mainly distributed in the cytoplasm. And in the cerebral cortex positive products were mostly concentrated in pyramidal cell-like neurons. In hippocampus, they were distributed in the molecular layer, the granular layer and the polymorphic cell layer. Additionally in the corpora quadrigemina and medulla oblongata positive products were concentrated in the neuronal. Moreover, the positive products of HIF1α and Beclin1 were distributed in the cerebellar Purkinje cells, and also presented in a small amount of cells in the granular layer and the molecular layer. The above results suggested that hippocampus and cerebral cortex might be susceptible to hypoxia, and HIF1α might promote Beclin1 expression and improve brain tissues adaptation to hypoxia. But the specific mechanism needs further studying. Therefore, this study provides theoretical basis for further exploration of brain tissues hypoxia adaptation mechanisms.

Key words： Yak Brain tissue Hypoxia inducible factor 1&#x003b1 (HIF1&#x003b1 ) Beclin1

Received: 10 February 2019

ZTFLH:

S852.1

Corresponding Authors: hejf@gsau.edu.cn; zq880204@126.com

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	Articles by authors
	BAI Zhan-Chun
	CUI Yan
	YU Si-Jiu
	Ma Jun-Xing
	HE Jun-Feng
	ZHANG Qian

Cite this article:

BAI Zhan-Chun,CUI Yan,YU Si-Jiu, et al. Expression and Distribution of HIF1α and Beclin1 in Adult Yak (Bos grunniens) Brain Tissues[J]. 农业生物技术学报, 2019, 27(10): 1878-1884.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2019.10.018 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2019/V27/I10/1878

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