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| Genetic Diversity of Garlic(Allium sativum L.) Germplasm by Simple Sequence Repeats |
| Shu-xia CHEN, , , , , |
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Abstract In order to investigate the distribution of simple sequence repeats(SSR) loci of Chinese garlic germplasm and provide the basis for the cultivars identification, germplasm conservation, and genetic improvement, the cluster analysis, principal componet analysis and genetic diversity of 40 garlic(Allium sativum L.) cultivars were analyzed using six pairs of SSR primers. A total of 21 polymorphic loci among these materials and average 3.5 polymorphic loci per SSR primer were detected. The percentage of polymorphic loci was 56.76%; the mean effective number of alleles, the mean Nei's gene diversity and the mean Shannon's information index were 1.5551, 0.3414 and 0.5188, respectively. Results showed that 40 materials could be divided into 3 groups at the similarity coefficient level of 0.59, in which the first group consisted of 28 cultivars which included 3 subgroups at the similarity coefficient level of 0.73, and the second group consisted of 2 cultivars and the third group consisted of 10 cultivars which could be classified into 2 subgroups at the similarity coefficient level of 0.68. The result of principal components analysis was almost consistent with that of UPGMA clustering analysis. The change range of Shannon-Weaver information index was 0.0576~0.4179, which showed the garlic had abundant genetic diversity. The genetic relationship and genetic diversity of garlic germplasm can be assessed efficiently by SSR markers. This study provides basic information of SSR markers for Chinese garlic germplasm
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Received: 05 August 2011
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