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| Genetic Diversity and Fingerprinting of 50 Pigmented Potato (Solanum tuberosum L.) Genetypes with SSR Markers |
| 1, 1, 1, 2 |
1. Key Laboratory of Horticultural Plant Biology, Ministry of Education
2. Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University
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Abstract Pigments of potato tubers have been suggested the benefits for human beings health and have attracted interests in science which approach to their genetic improvement, and in fresh and industrial markets which have potentials of added-values. Little information is available for diversity of pigmented potato germplasms resulted in a bottle-neck of selection and enhancement of suitable parental materials for the potato breeding. In present research, SSR markers were employed to clarify the genetic diversity and to establish the fingerprinting of 50 pigmented potato(Solanum tuberosum L.) genotypes which were available in existed breeding programs. The results showed that 56 pairs of SSR primers having polymorphism were selected by screening over 200 SSR markers. A total of 236 alleles were identified, of which 230 were polymorphic and the ratio of polymorphism was as high as 97.46%, which indicated that the SSR markers selected were suitable for the genetic diversity clarification. The genetic similarity of the 50 tested materials ranged from 0.50 to 1.00, suggesting relative narrow genetic resources which they were derived from. The UPGMA cluster analysis indicated that all the materials could be assigned into three groups at genetic similarity of 0.63. Cluster Ⅰ contained 11 genotypes, 38 genotypes were assigned to Cluster Ⅱ while Cluster Ⅲ had only one genotype. ClusterⅠwas assigned into 2 subgroups at genetic similarity of 0.64, and cluster Ⅱwas assigned into 3 subgroups at genetic similarity of 0.67. Five pairs of SSR primer were successfully used to establish DNA fingerprints of the 50 tested materials by detecting 32 alleles. Marker StI005 alone could identify 32 genotypes, bi-marker combination of StI005/StI007 could detect 43 genotypes and tri-marker combination of StI005/StI007/S038 could distinguish 46 genotypes. The rest four cultivars, British Columbia and Congo as well as MacIntosh Black and Black Beauty, could be further identified by S072 and S038, respectively. All of the 50 pigmented potato materials were assigned to 44 genotypes, which indicated that some of them may have variant names. Use of the data in parental material selection is recommended.
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Received: 01 December 2011
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