农业生物技术学报

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农业生物技术学报 2004年 12卷 4期刊出日期：2004-07-20

研究论文

鸡γ干扰素对H5亚型禽流感疫苗的免疫增强作用

Effect of rFPV-IFN-γ as Immunopotentiator on Active Immunization
Induced by H5 Subtype Avian Influenza Vaccines in Chickens

贾立军刘秀梵** 张艳梅彭大新张如宽

2004, 12(4): 427-430 | doi: | Full text (HTML) (1 KB) |

PDF (162 KB) ( 318 )

摘要

Ac/Ds双元激活标签体系用于建立水稻突变体材料

A Two-component Ac/Ds Activation Tagging System in Rice
Genome for Mutant Development

陈双燕吴运荣吴平**

2004, 12(4): 369-373 | doi: | Full text (HTML) (1 KB) |

PDF (330 KB) ( 182 )

摘要

棉花DNA遗传转化系的农艺性状变异和SSR标记分析

Identification of Trait Variance in the Progenies of Gossypium
hirsutum Transferred from G. barbadense by SSR Markers

杜雄明潘兆娥孙君灵周忠丽庞保印

2004, 12(4): 380-385 | doi: | Full text (HTML) (1 KB) |

PDF (307 KB) ( 296 )

摘要

花生矮化病毒(PSV) Mi株系 RNA3全序列分析

Nucleotide Sequence Analyses of RNA3 of Peanut Stunt Virus Mi Strain

许泽永1 晏立英1 陈坤荣1 Marcel Prins2

2004, 12(4): 436-441 | doi: | Full text (HTML) (1 KB) |

PDF (315 KB) ( 302 )

摘要

侵染东方百合的黄瓜花叶病毒两个分离物
cp 基因克隆和进化分析

Cloning and Phylogenetic Analysis of cp Gene for Two
CMV Isolates Infecting Lilium cv. Oriental Hybrids

刘文洪1, 2 洪健2 ** 陈集双1 叶美琴3

2004, 12(4): 442-445 | doi: | Full text (HTML) (1 KB) |

PDF (290 KB) ( 174 )

摘要

海岛棉 GbRac1基因过量表达提高转基因
烟草离体叶片对赤星病的抗性

Over Expression of GbRac1 Gene in Transgenic Tobacco Enhances Disease Resistance to Alternaria alternata In Vitro

李为民王志兴贾士荣*

2004, 12(4): 353-356 | doi: | Full text (HTML) (1 KB) |

PDF (413 KB) ( 224 )

摘要

短小芽孢杆菌β-1,4-木聚糖酶基因在大肠杆菌中的高效表达

Overexpression of Bacillus pumilus β-1,4-Xylanase Gene (xynA )
in Escherichia coli

刘伟丰毛爱军祝令香乔宇于巍董志扬**

2004, 12(4): 455-459 | doi: | Full text (HTML) (1 KB) |

PDF (327 KB) ( 213 )

摘要

柿和君迁子SSR分析技术的建立

Establishment of SSR Technique of Diospyros kaki and D.lotus

郭大龙罗正荣**

2004, 12(4): 386-389 | doi: | Full text (HTML) (1 KB) |

PDF (255 KB) ( 385 )

摘要

体外消化过程中低分子量寡肽释放量与饲料品质的关系

Relationship Between Low Molecular Weight Peptide Releasing
Patterns And Feed Quality During In Vitro Enzymatic Hydrolysis

王丽娟马秋刚计成**

2004, 12(4): 416-421 | doi: | Full text (HTML) (1 KB) |

PDF (220 KB) ( 234 )

摘要

大规模RNA阵列验证水稻茎组织特异性高表达基因

Identification of Rice (Oryza sativa L.) Stem Specific Genes
Using Large-scale RNA Array Technology

于凤池1 董海涛1** 谭学林2 王晟1 徐绍忠2 李德葆1**

2004, 12(4): 357-362 | doi: | Full text (HTML) (1 KB) |

PDF (408 KB) ( 369 )

摘要

编码日本梨树(Pyrus pyrifolia )花粉类β-1,3-葡聚糖酶蛋白的
cDNA克隆与核苷酸序列

Cloning and Nucleotide Sequence of the cDNA Encoding a
β-1,3-Glucanase-like Protein Secreted from Growing
Pollen Tubes of Japanese Pear (Pyrus pyrifolia)

周跃钢1** Naoko Norioka2 李绍良2 Shigemi Norioka2

2004, 12(4): 374-379 | doi: | Full text (HTML) (1 KB) |

PDF (269 KB) ( 353 )

摘要

低温预处理和高温饥饿胁迫对冬小麦
小孢子胚胎发生和植株再生的影响

Effect of Cold, Heat Shock and Starvation on Microspore
Embryogenesis and Regeneration in Winter Wheat

王宏芝1 杨成民1 魏建华1 杨凤萍2

2004, 12(4): 390-395 | doi: | Full text (HTML) (1 KB) |

PDF (292 KB) ( 238 )

摘要

高赖氨酸蛋白质基因和bar基因导入水稻及转基因植株的检测

Transfer of High Lysine-rich Gene and bar Gene into Rice by
Microprojectile Bombardment and Detection of Their Transgenic Plants

王为民赵倩朱登云于静娟** 敖光明

2004, 12(4): 363-368 | doi: | Full text (HTML) (1 KB) |

PDF (317 KB) ( 303 )

摘要

兔出血症病毒NJ85株衣壳蛋白基因在原核系统中的高效表达

High Expression of the Capsid Protein Gene of the Chinese Early
Isolate NJ85 of Rabbit Hemorrhagic Disease Virus in Escherichia coli

王永山1,2 陆承平1* 周宗安2

2004, 12(4): 408-411 | doi: | Full text (HTML) (1 KB) |

PDF (295 KB) ( 254 )

摘要

运送鹅源新城疫病毒DNA疫苗的减毒沙门氏菌及其免疫原性

DNA Vaccine Against Newcastle Disease Virus of Goose Origin
Delivered by Attenuated Salmonella typhimurium and Its Immunogenicity for Mice

潘志明1 焦新安1,2** 张小荣2 张晓明1 黄金林1 万洪全2 刘秀梵2

2004, 12(4): 412-415 | doi: | Full text (HTML) (1 KB) |

PDF (306 KB) ( 287 )

摘要

利用Cre/loxp定位重组系统构建雄性不育基因
和恢复基因表达载体

Constructions of Male Sterility Gene and Fertility Restoring Gene
Expression Vectors by Cre/loxp Site-specific Recombination System

宋洪元1** 曹必好2 丁建刚1 雷建军2 宋明1

2004, 12(4): 396-400 | doi: | Full text (HTML) (1 KB) |

PDF (445 KB) ( 294 )

摘要

电脉冲激活对体外成熟猪卵母细胞卵裂率和囊胚率的影响

Effects of Electrical Activation on the Rates of Cleavage and
Blastocyst of Porcine In Vitro Maturation Oocytes

田见晖1** 刘国世1 曾申明1 朱士恩1 蔡元2 刘焕营1 张忠诚1 吴常信1

2004, 12(4): 401-407 | doi: | Full text (HTML) (1 KB) |

PDF (258 KB) ( 294 )

摘要

家蚕茧色限性品种雌雄绢丝腺组织蛋白质组
双向凝胶电泳分析

Analysis on the Female and Male’s Middle Silkgland
Proteins of Cocoon Color Sex-limited Silkworm

靳远祥徐孟奎* 姜永煌陈玉银

2004, 12(4): 431-435 | doi: | Full text (HTML) (1 KB) |

PDF (255 KB) ( 312 )

摘要

通过导入几丁质酶基因提高荧光
假单胞杆菌P5的生防效果

Improving Biocontrol Effect of Pseudomonas fluorescence P5 on Plant Diseases through Genetic Modification with Chitinase Gene

徐小静1 张力群1** 朱有勇2 唐文华1

2004, 12(4): 460-463 | doi: | Full text (HTML) (1 KB) |

PDF (372 KB) ( 217 )

摘要

FAS 和HSL mRNA在猪背最长肌的表达
及其与肌内脂肪含量的关系

Expressions of FAS and HSL mRNA in Longissimus Dorsi
Muscle and Their Relation to Intramuscular Fat Contents in Pig

陈杰1，2 杨晓静1 佟辉1 陈杰1 赵茹茜1**

2004, 12(4): 422-426 | doi: | Full text (HTML) (1 KB) |

PDF (287 KB) ( 604 )

摘要

地衣芽孢杆菌β-1,3-1,4葡聚糖酶基因的克隆和表达

Cloning and Expression of β-1,3-1,4-glucanase Gene
from Bacillus licheniformis*

吕文平许梓荣** 杜文理李卫芬孙建义

2004, 12(4): 446-449 | doi: | Full text (HTML) (1 KB) |

PDF (301 KB) ( 269 )

摘要

曲霉Aspergillus sojae壳聚糖酶的诱导及特性

Induction and Some Properties of Chitosanase
from Aspergillus sojae

张学昆李加纳陆合

2004, 12(4): 450-454 | doi: | Full text (HTML) (1 KB) |

PDF (269 KB) ( 479 )

摘要

研究简报

短沟对虾和凡纳对虾溶菌酶cDNA的克隆与序列分析

Cloning and Sequencing of Lysozyme cDNAs of Penaeus semisulcatus and Litopenaeus vannamei

郑清梅叶星** 白俊杰吴锐全劳海华罗建仁

2004, 12(4): 482-483 | doi: | Full text (HTML) (1 KB) |

PDF (187 KB) ( 317 )

摘要

Chi 基因导入对转Bt棉花抗病虫性的影响

Influence of Chi Gene on Cotton Insect and Disease Resistance

简桂良1 卢美光1 王志兴2 贾士荣2 邹亚飞1 程红梅2 孙文姬1

2004, 12(4): 478-479 | doi: | Full text (HTML) (1 KB) |

PDF (141 KB) ( 237 )

摘要

瞬时表达黄瓜花叶病毒部分复制酶基因和番茄花叶病毒
移动蛋白基因的dsRNA能阻止相关病毒的侵染

Transient Expression of dsRNA of CMV Partial Replicase or ToMV Movement
Protein Gene Causes Interference with Homologous Virus Infection

牛颜冰于翠张凯崔晓峰陶小荣周雪平**

2004, 12(4): 484-485 | doi: | Full text (HTML) (1 KB) |

PDF (202 KB) ( 388 )

摘要

人溶菌酶基因的克隆及其在真核菌株中表达质粒pPIC9K/hLZM 的构建

Cloning of the Human Lysozyme Gene and Construction of the Expression Plasmid pPIC9K/hLZM in Eukaryotes Strain

王颖1 郝彦玲2 罗云波2

2004, 12(4): 480-481 | doi: | Full text (HTML) (1 KB) |

PDF (170 KB) ( 227 )

摘要

马铃薯AP1抗病传导链中蛋白元件的鉴定

Identification of Potato Protein Elements Interacting with AP1
　in Resistance Disease Conduction Cascade

冯洁徐进何礼远

2004, 12(4): 476-477 | doi: | Full text (HTML) (1 KB) |

PDF (170 KB) ( 129 )

摘要

综述

人乳铁蛋白转基因研究进展

Study Progress of Human Lactoferrin Transgene

赵春江刘兆良樊宝良李宁**

2004, 12(4): 470-475 | doi: | Full text (HTML) (1 KB) |

PDF (242 KB) ( 441 )

摘要

生物固氮研究中的蛋白质组学

Proteomics in Biological Nitrogen Fixation

韩威威沈世华荆玉祥**

2004, 12(4): 464-469 | doi: | Full text (HTML) (1 KB) |

PDF (496 KB) ( 599 )

摘要