Abstract:Abstract: The losing of varietal resistance to yellow rust(Puccinia striiformis Westend f. sp. tritric ) is an important factor causing periodical and massive yellow rust outbreak in wheat growing area. Emergency and development of new isolates of yellow rust pathogen have put widely planted varieties in China into a serious crisis of resistance losing and therefore put searching for new resistance resources into great need. Molecular marker technology could facilitate the identification and location of novel genes. The current study was undertaken using SSR technology in order to identify a novel yellow rust resistance gene derived from Spelt wheat (Triticum spelta L.) and study its chromosomal location. After screening 150 wheat microsatellite primers, a marker Xgwm155-147 bp located on chromosome 3A linked to this gene with the genetic distance of 40.5 cM was found, which indicated that the resistance gene is located on chromosome 3A. Since the only yellow rust resistance gene Yr5 known to be derived from Spelt is located on chromosome 2BL, the resistance gene identified in this study might be a novel yellow rust resistance gene which is temporarily designated YrSp.