Abstract：Abstract：High-molecular-weight glutenin subunits (HMW-GS) are main factors that affect bread-making quality of common wheat. Many common wheat cultivars derived from the Thinopyrum germplasm have good bread-making quality. But HMW-GS coded by E-genome of Thinopyrum was not reported previously. In the paper, the HMW-GS compositions of Thinopyrum elongatum (2n=14, EE) were analyzed by SDS-PAGE technique with materials of wheat (Triticum aestivum) var. Chinese Spring (CS), Thinopyrum elongatum (PI547326), CS-Th. elongatum amphidiploids, alien disomic and ditelosomic addition (DA) and alien disomic substitution (DS) lines. Studies on chromosomal location of HMW-GS gene of E-genome was performed in the meantime, and works of amplifying the HMW-GS gene of E-genome was also conducted by using PCR primers specific for the repetitive region of y-type HMW-GS genes. The main results were showed bellow: 1. E-genome in CS background coded one single HMW-GS with a highly similar migration ratio to subunit 1By8 of CS in SDS-PAGE gel, and in the accession of Thinopyrum elongatum PI547326 coded one subunit similar to 1By9 of Chenny. These two subunits were therefore temporarily named 1E8 and 1E9 respectively. 2. The HMW-GS gene of E-genome was located in the long arm of chromosome 1E. This result was consistent with the fact that the arrangement of HMW-GS genes was collinear in chromosomes of grain crops. 3. With primers for HMW-GS genes, one specific fragment in the approximately length of 1 300 bp was amplified in all materials containing 1EL chromosome. This fragment could be used as molecular marker for the HMW-GS gene of E-genome.
唐朝晖 刘守斌 尤明山 李保云 毛善锋 宋建民 刘广田. 普通小麦背景中长穗偃麦草高分子量麦谷蛋白基因的表达、 染色体定位与分子标记[J]. , 2003, 11(1): 34-39.
Tang Zhaohui Liu Shoubin You Mingshan Li Baoyun Mao Shanfeng Song Jianmin Liu Guangtian. Expression, Chromosomal Location and Molecular Marker of High-Molecular-Weight Glutenin Subunit Gene of Thinopyrum elongatum in Wheat Background. , 2003, 11(1): 34-39.
