摘要摘要：首次报道了从谷子(Stetaria italica )未成熟种子cDNA文库中克隆到1个与其它物种脂转移蛋白（LTP）具有较高同源性的脂转移蛋白基因Siltp。将核苷酸序列和推断的氨基酸序列在GenBank中进行比较，发现Siltp与大麦脂转移蛋白LTP7a2b和LTPcw-19,玉米脂转移蛋白，欧洲油菜的脂转移蛋白基因的核苷酸序列的同源性分别为72%,70%,60%,54%；氨基酸序列的同源性分别为79%,73%，65%，57%。序列分析表明，编码区含363个碱基 ，编码121个氨基酸，其中包括26个氨基酸的信号肽序列，分子量为9.3 kD。基因组Southern杂交结果表明，Siltp在基因组中以单拷贝存在。Northern杂交研究Siltp的时空表达特异性，发现Siltp仅在茎、叶、穗中表达。利用软件PAUP对17种植物的LTP进行进化分析，从进化系统树可以看出LTP有4个分支，其中，小麦发生分化较早，其它几种单子叶植物如玉米、谷子、大麦和水稻分化较晚，说明发生了早期的基因复制事件。在整个系统图中，双子叶比较分散，说明LTP基因的进化是个复杂的过程；禾本科作物的脂转移蛋白基因是由一个原始基因分化而产生的不同分支，Siltp与大麦LTP亲缘关系最近。
Abstract：Abstract: A cDNA encoding lipid transfer protein (Siltp ) was isolated from Stetaria italica cDNA library. According to the results of Blastn and Blastp in GenBank, it shares 72%,70%,60%,54% nucleotide sequence identity and 79%,73%，65%,57% amino acid sequence identity with Hordeum vulgare lipid transfer protein gene LTP7a2b and LTPcw-19, Zea mays ltp and Brassica napus ltp separately. It was sequenced and characterized. Siltp contains an open reading frame of 363 bp, 121 amino acid. The first 26 amino acid is supposed to be a signal sequence. The mature protein has a molecular mass of 9.3 kD. Southern blotting analysis indicated that Siltp is one copy in the genome. Siltp expresses in stem, leaves and grains. Using software PAUP to analyze evolution of LTP from 17 different plants, phylogenetic tree allows the conclusion that LTP has four groups. Differentiation happened earlier in wheat，while in the other monocots such as maize， millet，barley and rice happened later. It shows the early gene replicate matters. Dicotyls scatter in phylogenetic tree which illuminates the evolution of LTP gene is complex. It shows LTP gene of corns evolved and divided from one ancestral LTP gene. Siltp has most identity with barley LTP in the phylogenetic tree.
