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菜粉蝶提取物诱导黄瓜抗炭疽病相关基因cDNA片段的分离与分析
田菲菲1,张凤国1,朱杰华1,孙明清2,蒋继志3
1. 河北农业大学
2. 石家庄市农业技术推广中心
3. 河北大学
The Separating and Analysis of cDNA Fragments Related to Induced Resistance of Cucumber to Anthracnose by Pieris rapae Extract
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摘要 摘要:采用mRNA差异显示技术,以菜粉蝶提取物作为诱导剂,研究黄瓜幼苗抗炭疽病相关基因的差异表达,共得到67条差异表达基因片段。选其中5条被诱导表达的差异片段(D1、D2、D3、D4、D5)进行克隆、测序。生物信息学分析表明:D1片段序列(344bp)与来自叶绿体psbB基因编码的47kD蛋白结合叶绿素a构成的蛋白复合物photosystemIICP47同源性高达95.0%,其可能具有类似CP47的反复折叠的跨膜蛋白,在系统获得抗性信号途径中介导细胞壁和细胞质之间的信号传导;其余片段与已登记的基因同源性较低。
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田菲菲
张凤国
朱杰华
孙明清
蒋继志
关键词 菜粉蝶提取物黄瓜炭疽病诱导抗性mRNA差异显示    
Abstract:Abstract: The expressed gene in cucumber seedling leaf, which was inoculated with Colletotrichum orbiculare after Pieris rapae extract treatment and only was inoculated with C. orbiculare, were analysed by differential display reverse transcript polymerase chain reaction (DDRT-PCR). Sixty-seven differential fragments were obtained and five induced gene fragments were cloned and sequenced. Bioinformatics analysis revealed that the cDNA fragments D1 (344bp) had 95.0% homology to the photosystemIICP47 protein compound that was composed of chlorophyll a and the protein (47kD) encoded by psbB gene. It may had the transmembrane protein like CP47 and played an important role in the signal transmit of the systemic acquired resistance to anthracnose in cucumber. The other four fragments had lower homology to the gene segments registered in GenBank.
收稿日期: 2007-08-03     
通讯作者: 田菲菲   
引用本文:   
田菲菲1,张凤国1,朱杰华1,孙明清2,蒋继志3. 菜粉蝶提取物诱导黄瓜抗炭疽病相关基因cDNA片段的分离与分析[J]. , 2009, 17(4): 0-.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2009/V17/I4/0
 
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