Abstract:Abstract: The expressed gene in cucumber seedling leaf, which was inoculated with Colletotrichum orbiculare after Pieris rapae extract treatment and only was inoculated with C. orbiculare, were analysed by differential display reverse transcript polymerase chain reaction (DDRT-PCR). Sixty-seven differential fragments were obtained and five induced gene fragments were cloned and sequenced. Bioinformatics analysis revealed that the cDNA fragments D1 (344bp) had 95.0% homology to the photosystemIICP47 protein compound that was composed of chlorophyll a and the protein (47kD) encoded by psbB gene. It may had the transmembrane protein like CP47 and played an important role in the signal transmit of the systemic acquired resistance to anthracnose in cucumber. The other four fragments had lower homology to the gene segments registered in GenBank.