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2025年8月20日 星期三
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一个在超饲养朗德鹅肝脏中差异表达基因的分离和鉴定
赵阿勇1;何瑞国2**;汤华淳2;周圻1;汪海峰1
1.浙江林学院林业与生物技术学院,杭州,311300;2.华中农业大学动物科技学院,武汉,430070
Identification of a Differentially Expressed Gene in Fatty Liver of Overfeeding Landes
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摘要 为阐明鹅(Anser anser)肥肝形成的机制,使用mRNA差异显示技术研究超饲养和正常饲养条件下朗德鹅肝脏基因表达差异。基因转化生长因子β2(TGFB2)被证实在肥肝中上调(P < 0.01),该基因1 248 bp的cds序列(GenBank 登陆号EF541127)与鸡TGFB2有94%的同源性。序列分析表明,该序列含有一个1 239 bp的开放读码框架(ORF),编码412个氨基酸的蛋白质,该蛋白质序列存在2个保守的功能域:引导序列(TGFb前肽)和功能结构域(TGFβ),并与其它同源蛋白有较高的同源性。应用生物信息学方法对该蛋白质的功能和结构进行了分析。组织表达分析表明,鹅TGFB2在肝、肌胃、脾和卵巢中表达丰富,在子宫、肺和肌肉中中等表达,在肾和腹脂中表达量较低。结果显示,超饲养诱导了鹅肝脏TGFB2基因mRNA表达水平的上调。
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赵阿勇
何瑞国
**
汤华淳
周圻
汪海峰
关键词 肥肝 mRNA差异显示 TGFB2 荧光定量PCR Northern blot    
AbstractTo obtain understanding of its fattening mechanism, mRNA differential display reverse transcription PCR (DDRT-PCR) was applied to study the differences of gene expression in French Landes grey goose in conditions of overfeeding and normal feeding. One gene was found to be significantly up-regulated in fatty liver(P < 0.01), which had a cds length of 1 248 bp (GenBank accession No.EF541127) and showed 94% identity to chicken  transforming growth factor beta 2 (TGFB2). The sequence analysis revealed that its 1 239 bp-in-length open reading frame (ORF) encoded a 412-amino-acid protein containing two putative conserved domains of TGFb-propeptide and TGF-beta, and had high homology with its homologues. The function of this protein had been briefly reviewed based on published information. The tissue expression analysis indicated that goose TGFB2 mRNA was expressed higher in liver, muscular stomach, spleen and ovary than that in other tested tissues. The present results suggested that overfeeding could increase the mRNA expression level of goose TGFB2.
Key wordsfatty liver    mRNA differential display    TGFB2    Real-time quantitative PCR    Northern blot
收稿日期: 2008-06-03     
通讯作者: 何瑞国   
引用本文:   
赵阿勇1;何瑞国2**;汤华淳2;周圻1;汪海峰1. 一个在超饲养朗德鹅肝脏中差异表达基因的分离和鉴定[J]. , 2009, 17(2): 256-262.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/      或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2009/V17/I2/256
 
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