Abstract：Activation tagging is very important in plant genomics study. In this paper, an activation tagging library containing independent 30000 Basta-resistance lines was constructed by Agrobacterium tumefaciens-mediated transformation which used Arabidopsis bzr1-D as materials. Among these transformants, 47 lines showed obvious phenotype which is different from bzr1-D, containing late flowering time、dwarf、different shape of leaf、longer leaf petiole、trichomeless leaf 、sterile and no kink between stem/leaf. some T-DNA flanking sequences were obtained by Tail-PCR and Nested PCR which is the base for cloning mutant gene and co-segregation analysis.
曹冬梅 范喜英 王云山 康黎芳. 拟南芥激活标签突变体库的构建及突变体表型的分析[J]. , 2008, 16(2): 0-.
. Construction of an Activation Tagging Library of Arabidopsis and phenotype analysis of mutants. , 2008, 16(2): 0-.
