Abstract：Four breeds of chickens (White Leghorn, Yangshan, Taihe Silkies, White Recessive Rocks) with different egg production were applied to screen potential haplotype related to reproduction trait in part region of prolactin gene based on DNA pooling and sequencing. Two common haplotypes, CCCG and TGGA, of the most three ones CCCG, TGGA and TGCA which consisted with four SNPs sites (C-2402T, C-2161G, C-2062G and G-2040A) were found successfully in the fragment of 1028bp at the distal part of 5′flanking region. CCCG could be considered favourable for egg production since its frequency was estimated as 1 in White Leghorn which was verified by the results of PCR-RFLP and PCR-SSCP. Its effect on egg production was tested in the chicken reference of Nongdahe × Taihe Silkies, higher egg production was found in the haplotype combinations with CCCG, but no significant differences between them and the other haplotype combinations. The haplotype H3 (CCTCTG) which resulted from CCCG combining the other two sites (T-2101 and T-2054) was associated with egg production significantly in the chicken reference of Nongdahe × Taihe Silkies, and could be considered as a candidate marker for egg production. So rapidly screening potential causative haplotype could be succeeded applied DNA pooling and sequencing based on our researches.
