Abstract：In order to investigate the role of granulin(GRN) during the development of adipocyte, we constructed lentivirus interfering vector targeting on porcine GRN, packaged and infected the pig preadipocyte, differentiation state of preadipocyte was detected by Oil Red O staining and Oil Red O extraction assay, the expression levels of adipocyte differentiation marker genes were analysed by Real-time PCR. The results showed that the virus titer of the lentivirus reached 5×107 TU/mL, and the GRN expression level was remarkably decreased after lentivirus infection, of which, GRN mRNA expression was suppressed by approximate 76% by short hairpin RNA(shRNA2), moreover, adipocyte differentiation was promoted, and adipocyte differentiation marker genes, such as peroxisome proliferator activated receptor γ(PPARγ), sterol regulatory element binding protein 1(SREBP-1C), adipocyte-type fatty acid-binding protein(ap2) and lipoprotein lipase(LPL) mRNA expression levels were all increased. Taken together these data suggested that knocking down of GRN promoted adipocyte differentiation, and GRN may play a negative role in pig preadipcoyte differentiation.
