Abstract：To establish DNA database for individual identification and parentage analysis system in Chinese Hostein bulls, eleven microsatellites(BM1824、BM2113、ETH3、ETH10、ETH225、INRA023、SPS115、TGLA53、TGLA122、TGLA126 and TGLA227)recommend by International Society of Animal Genetics(ISAG) and four microsatellites(MAF45、MCM158、UNM0108 and UMN0929) selected in Y chromosome were used to examine 2 150 Holstein bulls(Bos taurus) with fluorescence primer PCR and genetic analysis equipment of ABI3130, discussing feasibility on individual identification and parentage testing in cattle. Results showed that mean of heterozygosity was 0.766 and polymorphism information content(PIC) with 0.738 in 15 microsatellites markers. Largest mean of PIC in MCM158 marker was 0.866, and lowest mean of PIC in SPS115 marker was 0.588, belonging to high polymorphism markers. Combined power of discrimination, combined power of exclusion and probability of identity of 15 microsatellites markers were 99.99%, 99.99% and 1.62×10-17, respectively. Therefore, effectiveness and reliability of 15 microsatellites markers were high for individual identification and parentage analysis. In addition, DNA database for individual and parentage identification was established in this study, and progress was made in the standardization, normalization and simplification for individual identification and parentage analysis.
