摘要本文以黄牛卵母细胞胞质为受体，牦牛耳成纤维细胞为核供体进行体细胞核移植，研究供体细胞性别、细胞周期同期化处理方式及冷冻对牦牛异种克隆胚胎发育的影响。结果显示：雄性供体细胞的牦牛异种克隆囊胚发育率显著高于雌性供体细胞（56.6% vs 39.5％，P﹤0.05），但卵裂率和囊胚细胞数差异不显著；血清饥饿和接触抑制提供的供体细胞对牦牛异种克隆胚胎发育率和胚胎质量的影响没有明显差别；供体细胞冷冻后解冻组的克隆胚胎卵裂率明显低于新鲜消化组（54.5％ vs 78.2％，P﹤0.05），但两组之间的囊胚率和囊胚细胞数没有显著差异。说明供体细胞的性别对牦牛异种克隆囊胚发育有影响，而同期化处理和细胞冷冻对牦牛异种克隆囊胚发育影响很少。
Abstract：The purpose of this study was to evaluate the effects of donors’ sex, cell cycle synchromization treatments and donor nuclei obtained from fresh or frozen-thawed on in vitro developmental competence of yak-bovine interspecies nuclear transfer(NT) embryos. In this paper, we used bovine oocytes as recipient, yak ear fibroblast cell as donor. The results indicated that the development rate of male blastocysts was higher than female（56.6% vs 39.5％，P﹤0.05）, whereas cleavage and total cell number had no difference between the two groups. No significant difference was observed in the blastocysts development and quality of donor treat with serum starvation or confluence inhibition. There was no significant difference in embryos development of use fresh or frozen-thaw donor cell, whereas cleavage in group of fresh donor cell was significantly higher than frozen-thaw group（54.5％ vs 78.2％，P﹤0.05）. Our results demonstrated that donors’ sex could impact the in vitro developmental competence of yak-bovine interspecies NT embryos, whereas different cell cycle synchromization treatment and freezing had little influence.
刘颖 朱士恩 李荣 王莉莉 王海萍 丁方荣 李京 李松 戴蕴平. 供体细胞不同来源和处理方式对牦牛异种克隆胚胎发育的影响[J]. , 2008, 16(1): 0-.
. Effects of donors’ sex and different cell treatments on in vitro development of yak-bovine interspecies nuclear transfer embryos. , 2008, 16(1): 0-.
