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维生素A对高脂饲喂小鼠脂肪降解及胆固醇清除的影响
堵斌1,孙超2,谢亮1,彭永佳1,解云菲3
1. 西北农林科技大学动物科技学院
2. 西北农林科技大学
3.
Effects of Vitamin A on Cholesterol Cleaning and Stearolysis of High-fat Diet Fed Mice
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摘要 摘 要 研究维生素A(VA)对高脂饲喂条件下小鼠(Mus musculus)肝脏中脂肪降解相关基因mRNA表达和血清胆固醇含量的影响,探索其是否可促进小鼠体内脂肪降解及胆固醇清除。通过石蜡切片观察高脂饲喂小鼠肝脏中脂滴分布;利用Real-time PCR检测小鼠肝脏各相关基因mRNA表达量;采用试剂盒测定血清胆固醇含量。结果显示,第1 ~ 3天VA处理组与对照组小鼠体重差异不显著(P>0.05);第4、9、27天,VA处理组小鼠体重显著低于对照组(P<0.05);VA处理组皮下脂肪(0.1890 ±0.0056) g与腹膜下脂肪垫(0.3862 ±0.0053) g重量极显著低于对照组(0.2620 ±0.0020) g与(0.4867 ±0.0052) g重量(P<0.01);切片观察肝脏充脂细胞数量明显减少;VA处理组小鼠肝脏清道夫受体B族Ⅰ型( (SR-BⅠ)、激素敏感脂酶 (HSL)基因mRNA表达量极显著高于对照组(P<0.01);过氧化物酶体增生物激活受体γ (PPARγ) 、固醇调节元件结合蛋白-1c (SREBP-1c)基因表达量显著低于对照组(P<0.05);甘油三酯水解酶 (TGH)基因表达量显著高于对照组(P< 0.05);VA处理组小鼠血清胆固醇 (CHO)及高密度脂蛋白胆固醇 (HDL-C)浓度均极显著低于对照组CHO及HDL-C浓度(P<0.01)。说明VA可通过SR-BⅠ促进脂肪降解和血清中HDL-C清除。
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堵斌
孙超
谢亮
彭永佳
解云菲
关键词 维生素A小鼠高脂饲喂HDL-CSR-BI    
Abstract:Abstract In order to explore the role of vitamin A(VA) on fatty deposition and cholesterol cleaning, the distribution of hepatic lipid droplet in high-fat diet fed mouse(Mus musculus) was observed by paraffin section preparation methods; the relative level of hepatic stearolysis related gene mRNA was determined by real-time RT-PCR and serum cholesterol(CHO) and high density lipoprotein-cholesterol(HDL-C) levels. Results showed that: first three days there was no significant difference between VA treated and control group on the daily gain(P>0.05); on the 4th, 9th and 27th day the daily gain was significantly lower than that of control group (P<0.05); the subcutaneous fat and fat pad under peritoneum were significantly lower in VA treated (0.1890 ±0.0056) g and (0.3862 ±0.0053) g than that of control group (0.2620 ±0.0020) g and (0.4867 ±0.0052) g (P<0.01); the amount of hepatic lipid droplet was fewer in VA treated than that of control group; the expression levels of scavenger receptor-BⅠ(SR-BⅠ) and hormonesensitive lipase(HSL) mRNA were significantly higher in VA treated than that of control group(P<0.01); peroxisome proliferators activated receptor γ(PPARγ) and sterol regulatory element binding protein-1c (SREBP-1c) were significantly lower in VA treated than that of control group(P<0.05); triglyceride hydrolyase(TGH) mRNA was significantly higher in VA treated than that of control group(P<0.05); and the levels of serum CHO and HDL-C were significantly lower in VA treated than that of control group(P<0.01). Our results suggest that VA profits to fat degradation and cholesterol cleaning.
Key wordsVitamin A    Mice    High-Fat Diet    HDL-C    SR-BI
收稿日期: 2009-02-20     
通讯作者: 堵斌   
引用本文:   
堵斌1,孙超2,谢亮1,彭永佳1,解云菲3. 维生素A对高脂饲喂小鼠脂肪降解及胆固醇清除的影响[J]. , 2010, 18(4): 771-776.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2010/V18/I4/771
 
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