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鸭A-FABP基因内含子1克隆和序列多态性分析
张海波1,徐琪2,张依裕3,段修军3,陈国宏2
1. 扬州大学
2. 扬州大学动物科学与技术学院
3.
Cloning of Duck A-FABP Gene Intron-1 and Analysis of Gene Polymorphism
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摘要 摘要:根据鸭A-FABP mRNA序列和鸡A-FABP基因组序列设计1对引物扩增鸭A-FABP基因内含子1序列,同时根据扩增产物设计4对引物,利用PCR-SSCP对鸭A-FABP基因内含子1和外显子2进行多态性研究,对不同鸭群体进行群体遗传学分析。结果表明:克隆序列包含鸭A-FABP基因外显子1、2部分序列和完整的内含子1序列,与鸡A-FABP基因内含子1同源性为75.1%;经SSCP检测,发现引物P1扩增片段有3处碱基突变,引物P3有6处碱基突变,这些突变分别产生了3种和8种基因型。在P1位点樱桃谷鸭和苏牧麻鸭偏离了Hardy-Weinberg平衡(P<0.01),金定鸭基因型分布符合Hardy-Weinberg平衡(P>0.05),在P3位点3个群体均符合Hardy-Weinberg平衡(P>0.05)。同时,这些基因型在不同种群中分布存在显著或及显著差异(P<0.05或P<0.01)。群体遗传学分析结果显示A-FABP基因在不同鸭群体中具有丰富的单核苷酸多态性,可以进一步作为候选基因来分析其与脂肪性状相关性。
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张海波
徐琪
张依裕
段修军
陈国宏
关键词 A-FABP克隆SSCP    
Abstract:Abstract: A pair of primers P-in1 were designed according to the sequences of Adipocyte Fatty Acid-Binding Protein (A-FABP) gene mRNA in duck and A-FABP gene in chicken to amplified duck A-FABP intron-1, then 4 pairs of primers were designed according to cloned gene. The SNPs were detected by technique of PCR-SSCP, confirmed by sequencing and to study the. genetic discrepancy in different groups. The results indicated that the product of P-in1 included partial exon-1, exon-2 and completely intron-1 after cloned and sequenced and homologous comparison was done between duck and chicken A-FABP gene, the results showed the homology was 75.1%. Three and six nucleotides mutation were found in the products of primer P1 and P3, which resulted in 3 and 6 genotypes, separately. The results of Chi-Square test showed that Cherry Valley duck and Sumu duck deviated from the Hardy-Weinberg equilibrium (P<0.01) except Jingding duck in P1 locus , but all of these three groups fit with it in P3 locus (P>0.05) and the distribution of them was different or very different in various groups (P<0.05 or P<0.01).Genetic analysis showed there had a high level of polymorphism in different duck groups, and could be used as a candidate gene to analysis the correlation between its polymorphism and fat traits in duck.
Key wordsduck    A-FABP    clone    SSCP
收稿日期: 2009-01-18     
通讯作者: 陈国宏   
引用本文:   
张海波1,徐琪2,张依裕3,段修军3,陈国宏2. 鸭A-FABP基因内含子1克隆和序列多态性分析[J]. , 2010, 18(1): 75-80.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2010/V18/I1/75
 
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