Abstract：Resistin play important roles in organism lipometabolism and energy metabolism. The present work was used to study the effects of resistin on proliferation and differentiation of porcine preadipocyte. In this paper, the preadipocytes were isolated from subcutaneus adipose tissue of 1-day-old crossbred piglet, and the preadipocytes were respectively cultivated in the DMEM/F12 culture media containing 0, 50, 100, 150 and 200 μg/L recombinant resistin. The changes of the cell morphology were observed, and the triglyceride content and the activity of lipoprotein lipase of the cells were determined to evaluate proliferation and differentiation of porcine preadipocyte. Results showed that the proliferation of porcine preadipocytes were significantly promoted by 0 to 100 μg/L resistin supplementation in the media, the activity of lipoprotein lipase of the cells was also significantly increased. As the resistin concentration in the culture media came to 100~200 μg/L, the facilitory effect of resistin on proliferation of porcine preadipocyte was declined. Moreover, as resistin concentration increased, the differentiation of porcine preadipocyte was significantly strengthened, the intracellular triglyceride synthesize and fatty droplet aggregation were promoted simultaneously. The results indicated that the resistin has dual effects on growth of porcine preadipocyte.
