Abstract：The new DNA extraction method we established was based on the traditional alkaline lysis method. We changed the volume of neutralization solution and introduced an isopropanol precipitated step. The result showed that DNA extracted by this method can amplify target genes fragments which were short than 600 bp. Only 1% expected material contained in other samples can generate the specific amplified. The whole process can be finished in 21 minutes, and the applicability of NaOH-based method is extended.
许文涛. 一种适于PCR扩增的植物基因组快速提取新方法的研究[J]. , 2010, 18(2): 394-399.
韦露450103198306212562李丽婷120103198310162928. A new simple and rapid plant DNA extraction method for PCR analysis. , 2010, 18(2): 394-399.
